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Title: 逆相系高速液体クロマトグラフィーによる高度不飽和脂肪酸含有トリグリセリド分子種の分離改善に及ぼすカラム温度の影響
Other Titles: Effect of Column Temperature on Improvement of Resolution in Separating Triglyceride Molecular Species Containing Highly Unsaturated Fatty Acids by Reverse Phase High Performance Liquid Chromatography
Authors: 澤田, 哲志1 Browse this author
高橋, 是太郎2 Browse this author →KAKEN DB
羽田野, 六男3 Browse this author →KAKEN DB
Authors(alt): Sawada, Tetsushi1
Takahashi, Koretaro2
Hatano, Mutsuo3
Issue Date: 1992
Publisher: 日本水産学会
Journal Title: 日本水産学会誌
Journal Title(alt): Bulletin of the Japanese Society of Scientific Fisheries
Volume: 58
Issue: 7
Start Page: 1313
End Page: 1317
Publisher DOI: 10.2331/suisan.58.1313
Abstract: By taking advantage of the low melting point of fish oil, chilled-column reverse-phase high-performance liquid chromatography (HPLC) was examined in order to obtain good resolution of triglycerioe (TG) molecular species. TGs containing high proportions of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were synthesized by using 90% pure EPA and DHA with a bacterial lipase. Four TG molecular species thus obtained were subjected to HPLC analysis, from which the difference in retention time, theoretical plate number, and resolution of the adjacent peaks were discussed in relation to the column temperature of the system. The lower the column temperature, the better the theoretical plate number and resolution. However, they reached a maximum at around -15°C. It was concluded that low temperature, wherever it is practicable not to exceed the melting point, is the best temperature for fish oil TG molecular species analysis and fractionation
Rights: © 1992 公益社団法人日本水産学会
© 1992 The Japanese Society of Fisheries Science
Type: article
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 是太郎

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