Detection and identification of infectious hematopoietic necrosis virus (IHNV) by reverse transcription (RT)-polymerase chain reaction (PCR)

Yoshinaka, Toko; Yoshimizu, Mamoru; Sawabe, Tomoo; Ezura, Yoshio

doi:10.2331/fishsci.63.592


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Detection and identification of infectious hematopoietic necrosis virus (IHNV) by reverse transcription (RT)-polymerase chain reaction (PCR)

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Title:	Detection and identification of infectious hematopoietic necrosis virus (IHNV) by reverse transcription (RT)-polymerase chain reaction (PCR)
Authors:	Yoshinaka, Toko Browse this author
	Yoshimizu, Mamoru Browse this author →KAKEN DB
	Sawabe, Tomoo Browse this author →KAKEN DB
	Ezura, Yoshio Browse this author →KAKEN DB
Keywords:	IHNV
	RT-PCR
	fish-rhabdoviruses
	rhabdovirus
Issue Date:	1997
Publisher:	Japanese Society of Fisheries Science
Journal Title:	Fisheries science
Volume:	63
Issue:	4
Start Page:	592
End Page:	595
Publisher DOI:	10.2331/fishsci.63.592
Abstract:	A method based on reverse transcription (RT)-polymerase chain reaction (PCR) was established for detection and identification of infectious hematopoietic necrosis virus (IHNV). A set of primers was prepared for amplification of a 510 bp nucleotide which encodes a region of IHNV nucleoprotein (N) gene. The PCR product was confirmed as the IHNV specific nucleotide by southern hybridization with an oligonucleotide probe synthesized from the N gene. The PCR was able to amplify the target sequence from five representative strains of IHNV from Japan and North America. There was no PCR product from five other fish rhabdoviruses. Viruses from ovarian fluid of masu salmon collected at Shibetsu River, Ichani River in Hokkaido and kidney tissue of rainbow trout in Aichi Prefecture were isolated and identified using the RT-PCR.
Rights:	© 1997 公益社団法人日本水産学会
Rights:	© 1997 The Japanese Society of Fisheries Science
Type:	article
URI:	http://hdl.handle.net/2115/52089
Appears in Collections:	水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 澤辺智雄

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