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Growth suppression of the marine phytoplankton Alexandrium catenella and Tetraselmis sp. caused by ultrafiltrated seawater collected from coastal area on the mouth of Funka Bay

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Title: Growth suppression of the marine phytoplankton Alexandrium catenella and Tetraselmis sp. caused by ultrafiltrated seawater collected from coastal area on the mouth of Funka Bay
Authors: Onji, Masashi Browse this author
Sawabe, Tomoo Browse this author →KAKEN DB
Ezura, Yoshio Browse this author →KAKEN DB
Keywords: phytoplankton
Alexandrium catenella
Tetraselmis sp.
growth suppression
filtrable pathogens
Issue Date: 1999
Publisher: Japanese Society of Fisheries Science
Journal Title: Fisheries science
Volume: 65
Issue: 5
Start Page: 687
End Page: 693
Publisher DOI: 10.2331/fishsci.65.687
Abstract: Filtrable pathogens infecting the phytoplankton Alexandrium catenella and Tetraselmis sp. were screened from coastal seawater on the mouth of Funka Bay, Hokkaido, Japan from 1993 to 1994. Growth suppression against these phytoplankton species was observed in the seawater samples collected during September and October 1993. The growth of A. catenella was suppressed from 40 to 45%, and that of Tetraselmis sp. was suppressed from 20 to 30%. Re-inoculation of the culture filtrate of growth-suppressed phytoplankton after passing the culture through a 0.22-μm filter also caused growth suppression of the fresh culture. However, these effects disappeared after several treatments including heating at 50℃ for 30min, exposure to acidic conditions below pH 5.0, passing through a 0.05-μm filter, and Proteinase K and RNase treatment. Cell free extracts of the growth-suppressed phytoplankton caused the same extent of growth suppression. Electron microscopic observation of A. catenella cells that were lead to the growth suppression revealed that the cells were severely damaged, whereas no virus-like particles or bacterial cells were observed. Growth suppression was observed in a fresh culture of A. catenella and an axenic culture of Gymnodinium mikimotoi by the growth suppressed Tetraselmis sp. culture filtrate, and the A. catenella culture filtrate affected the growth of Tetraselmis sp. and an axenic culture of G. mikimotoi. However, the growth suppression or inhibition was not observed in fresh cultures of Prorocentrum micans, P. minimum, A. tamarense, G. mikimotoi, Chattonella antiqua, C. marina, and Heterosigma akashiwo. These results suggested that unique filterable pathogens might be found in the seawater samples.
Rights: © 1999 公益社団法人日本水産学会
© 1999 The Japanese Society of Fisheries Science
Type: article
URI: http://hdl.handle.net/2115/52092
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 澤辺 智雄

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