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Characteristics of virus-like growth suppression agents against phytoplankton obtained from seawater at the Mouth of Funka Bay, Hokkaido, Japan

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Title: Characteristics of virus-like growth suppression agents against phytoplankton obtained from seawater at the Mouth of Funka Bay, Hokkaido, Japan
Authors: Onji, Masashi Browse this author
Sawabe, Tomoo Browse this author →KAKEN DB
Ezura, Yoshio Browse this author →KAKEN DB
Keywords: Alexandrium catenella
growth suppression
Gymnodinium mikimotoi
proteinase sensitivity
Tetraselmis sp.
ultracentrifugation
Issue Date: 2000
Publisher: Japanese Society of Fisheries Science
Journal Title: Fisheries science
Volume: 66
Issue: 1
Start Page: 38
End Page: 43
Publisher DOI: 10.1046/j.1444-2906.2000.00005.x
Abstract: Eighteen samples of virus-like growth suppression agents against the marine phytoplankton Alexandrium catenella, Gymnodinium mikimotoi and Tetraselmis sp., obtained from the coastal waters at the mouth of Funka Bay, Hokkaido, Japan, from 1993 to 1995, were characterized on size estimation, heat stability, nuclease sensitivity, proteinase K sensitivity, stability under acidic condition, titration, diethyl ether sensitivity, and ultraviolet (UV) sensitivity. All agents were affected by heating at 50℃ for 30 min, exposure to acidic conditions below pH 5.0, passing through a 0.05 μm filter, RNase treatment, and irradiation of UV dosage at 5 × 10^4 μW/s per cm2. The growth suppression effects of 11 agents from Tetraselmis sp. and A. catenella disappeared after proteinase K treatment, however, seven agents from G. mikimotoi were unaffected by this treatment. Furthermore, 11 agents against Tetraselmis sp. and A. catenella were collected from the bottom fraction by ultracentrifugation, while seven agents against G. mikimotoi were collected from the upper fraction, not from the precipitated fraction. These results suggest that at least two types exist in the virus-like agents showing growth suppression for phytoplankton.
Rights: © 2000 公益社団法人日本水産学会
© 2000 The Japanese Society of Fisheries Science
Type: article
URI: http://hdl.handle.net/2115/52093
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 澤辺 智雄

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