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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Purification and characterization of an alginate lyase from marine Alteromonas sp.
| Title: | Purification and characterization of an alginate lyase from marine Alteromonas sp. |
| Authors: | Sawabe, Tomoo Browse this author →KAKEN DB | | Ezura, Yoshio Browse this author →KAKEN DB | | Kimura, Takahisa Browse this author →KAKEN DB |
| Issue Date: | 1992 |
| Publisher: | 日本水産学会 |
| Journal Title: | 日本水産学会誌 |
| Journal Title(alt): | Bulletin of the Japanese Society of Scientific Fisheries |
| Volume: | 58 |
| Issue: | 3 |
| Start Page: | 521 |
| End Page: | 527 |
| Publisher DOI: | 10.2331/suisan.58.521 |
| Abstract: | An alginate lyase from the culture medium of Alteromonas sp. H-4 was purified by ultrafiltra-tion, gel filtration, and anion-exchange chromatography and was characterized. A molecular weight of the purified enzyme was estimated as 32, 000 by sodium dodecyl sulfate-polyacrylamide gelectrophoresis (SDS-PAGE). Optimum pH and temperature of the enzyme activity were 7.5 and 30°C, respectively. The enzyme was unstable on heating and in acidic solution. The al-ginate lyase required 50mm MgCl2 or MgSO4, 0.5M NaCI, and 0.2M KCl for the maximum acti-vity. |
| Rights: | © 1992 公益社団法人日本水産学会 | | © 1992 The Japanese Society of Fisheries Science |
| Type: | article |
| URI: | http://hdl.handle.net/2115/52097 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 澤辺 智雄
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