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Purification and characterization of an alginate lyase from marine Alteromonas sp.

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/52097

Title: Purification and characterization of an alginate lyase from marine Alteromonas sp.
Authors: Sawabe, Tomoo Browse this author →KAKEN DB
Ezura, Yoshio Browse this author →KAKEN DB
Kimura, Takahisa Browse this author →KAKEN DB
Issue Date: 1992
Publisher: 日本水産学会
Journal Title: 日本水産学会誌
Journal Title(alt): Bulletin of the Japanese Society of Scientific Fisheries
Volume: 58
Issue: 3
Start Page: 521
End Page: 527
Publisher DOI: 10.2331/suisan.58.521
Abstract: An alginate lyase from the culture medium of Alteromonas sp. H-4 was purified by ultrafiltra-tion, gel filtration, and anion-exchange chromatography and was characterized. A molecular weight of the purified enzyme was estimated as 32, 000 by sodium dodecyl sulfate-polyacrylamide gelectrophoresis (SDS-PAGE). Optimum pH and temperature of the enzyme activity were 7.5 and 30°C, respectively. The enzyme was unstable on heating and in acidic solution. The al-ginate lyase required 50mm MgCl2 or MgSO4, 0.5M NaCI, and 0.2M KCl for the maximum acti-vity.
Rights: © 1992 公益社団法人日本水産学会
© 1992 The Japanese Society of Fisheries Science
Type: article
URI: http://hdl.handle.net/2115/52097
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 澤辺 智雄

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