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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Application of an alginate lyase from Alteromonas sp. for isolation of protoplasts from a brown algae Laminaria japonica
| Title: | Application of an alginate lyase from Alteromonas sp. for isolation of protoplasts from a brown algae Laminaria japonica |
| Authors: | Sawabe, Tomoo Browse this author →KAKEN DB | | Ezura, Yoshio Browse this author →KAKEN DB | | Kimura, Takahisa Browse this author →KAKEN DB |
| Issue Date: | 1993 |
| Publisher: | 日本水産学会 |
| Journal Title: | 日本水産学会誌 |
| Journal Title(alt): | Bulletin of the Japanese Society of Scientific Fisheries |
| Volume: | 59 |
| Issue: | 4 |
| Start Page: | 705 |
| End Page: | 709 |
| Publisher DOI: | 10.2331/suisan.59.705 |
| Abstract: | An alginate lyase from Alteromonas sp. H-4 was used for the preparation of protoplast from young sporophytes of Laminaria japonica. Optimum conditions for the isolation of protoplasts were as follows: a) fine pieces of the sporophytes chopped by a razor were incubated in an enzyme mixture for 3 to 5h at 15°C with shaking, b) the composition of the enzyme mixture was purified alginate lyase (30U/ml) with cellulase Onozuka R-10 (1.5%) in a hypertonic solution (0.7M mannitol, 25mM MgCl2, and 5mM HEPES in 50% seawater, pH7.8). Yields of protoplast were 106-107 cells/g (sporophytes) and protoplast viability was more than 75%. Protoplasts were brown-greenish and spherical with a diameter of 10-25μm. The purity of alginate lyase from the strain H-4 scarcely influenced the yield of protoplast, but purified enzyme produced the highest viability of cells. |
| Rights: | © 1993 公益社団法人日本水産学会 | | © 1993 The Japanese Society of Fisheries Science |
| Type: | article |
| URI: | http://hdl.handle.net/2115/52098 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 澤辺 智雄
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