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Application of an alginate lyase from Alteromonas sp. for isolation of protoplasts from a brown algae Laminaria japonica

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Title: Application of an alginate lyase from Alteromonas sp. for isolation of protoplasts from a brown algae Laminaria japonica
Authors: Sawabe, Tomoo Browse this author →KAKEN DB
Ezura, Yoshio Browse this author →KAKEN DB
Kimura, Takahisa Browse this author →KAKEN DB
Issue Date: 1993
Publisher: 日本水産学会
Journal Title: 日本水産学会誌
Journal Title(alt): Bulletin of the Japanese Society of Scientific Fisheries
Volume: 59
Issue: 4
Start Page: 705
End Page: 709
Publisher DOI: 10.2331/suisan.59.705
Abstract: An alginate lyase from Alteromonas sp. H-4 was used for the preparation of protoplast from young sporophytes of Laminaria japonica. Optimum conditions for the isolation of protoplasts were as follows: a) fine pieces of the sporophytes chopped by a razor were incubated in an enzyme mixture for 3 to 5h at 15°C with shaking, b) the composition of the enzyme mixture was purified alginate lyase (30U/ml) with cellulase Onozuka R-10 (1.5%) in a hypertonic solution (0.7M mannitol, 25mM MgCl2, and 5mM HEPES in 50% seawater, pH7.8). Yields of protoplast were 106-107 cells/g (sporophytes) and protoplast viability was more than 75%. Protoplasts were brown-greenish and spherical with a diameter of 10-25μm. The purity of alginate lyase from the strain H-4 scarcely influenced the yield of protoplast, but purified enzyme produced the highest viability of cells.
Rights: © 1993 公益社団法人日本水産学会
© 1993 The Japanese Society of Fisheries Science
Type: article
URI: http://hdl.handle.net/2115/52098
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 澤辺 智雄

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