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Draft Genome Sequence of a Psychrotolerant Sulfur-Oxidizing Bacterium, Sulfuricella denitrificans skB26, and Proteomic Insights into Cold Adaptation
| Title: | Draft Genome Sequence of a Psychrotolerant Sulfur-Oxidizing Bacterium, Sulfuricella denitrificans skB26, and Proteomic Insights into Cold Adaptation |
| Authors: | Watanabe, Tomohiro Browse this author | | Kojima, Hisaya Browse this author →KAKEN DB | | Fukui, Manabu Browse this author →KAKEN DB |
| Issue Date: | Sep-2012 |
| Publisher: | American Society for Microbiology |
| Journal Title: | Applied and Environmental Microbiology |
| Volume: | 78 |
| Issue: | 18 |
| Start Page: | 6545 |
| End Page: | 6549 |
| Publisher DOI: | 10.1128/AEM.01349-12 |
| Abstract: | Except for several conspicuous cases, very little is known about sulfur oxidizers living in natural freshwater environments. Sulfuricella denitrificans skB26 is a psychrotolerant sulfur oxidizer recently isolated from a freshwater lake, as a representative of a new genus in the class Betaproteobacteria. In this study, approximately 3.2 Mb of draft genome sequence of the strain skB26 was obtained. In the draft genome consisting of 23 contigs, a single rRNA operon, 43 tRNA genes, and 3,133 coding sequences were identified. The identified genes include those required for sulfur oxidation, denitrification, and carbon fixation. Comparative proteomic analysis was conducted to assess cold adaptation mechanisms of this organism. From cells grown at 22℃ and 5℃, proteins were extracted for analysis with nano-liquid chromatography-electrospray ionization-tandem mass spectrometry. In the cells cultured at 5℃, relative abundances of ribosomal proteins, cold shock proteins, and DEAD/DEAH box RNA helicases were increased in comparison to 22℃. These results suggest that maintenance of proper translation is critical for growth under low-temperature conditions, similar to other cold-adapted prokaryotes. |
| Rights: | © 2012 American Society for Microbiology |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/52110 |
| Appears in Collections: | 低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 小島 久弥
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