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pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells
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| Title: | pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells |
| Authors: | Islam, Md. Serajul Browse this author | | Honma, Masato Browse this author | | Nakabayashi, Takakazu Browse this author →KAKEN DB | | Kinjo, Masataka Browse this author →KAKEN DB | | Ohta, Nobuhiro Browse this author →KAKEN DB |
| Keywords: | FAD | | autofluorescence | | living cell | | fluorescence lifetime imaging | | intracellular pH | | fluorescence decay |
| Issue Date: | Jan-2013 |
| Publisher: | MDPI |
| Journal Title: | International Journal of Molecular Sciences |
| Volume: | 14 |
| Issue: | 1 |
| Start Page: | 1952 |
| End Page: | 1963 |
| Publisher DOI: | 10.3390/ijms14011952 |
| Abstract: | We have studied physiological parameters in a living cell using fluorescence lifetime imaging of endogenous chromophores. In this study, pH dependence of the fluorescence lifetime of flavin adenine dinucleotide (FAD), that is a significant cofactor exhibiting autofluorescence, has been investigated in buffer solution and in cells. The fluorescence lifetime of FAD remained unchanged with pH 5 to 9 in solution. However, the fluorescence lifetime in HeLa cells was found to decrease with increasing intracellular pH, suggesting that pH in a single cell can be estimated from the fluorescence lifetime imaging of FAD without adding exogenous fluorescent probes. |
| Rights: | http://creativecommons.org/licenses/by/3.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/52120 |
| Appears in Collections: | 電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 太田 信廣
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