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pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells

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Title: pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells
Authors: Islam, Md. Serajul Browse this author
Honma, Masato Browse this author
Nakabayashi, Takakazu Browse this author →KAKEN DB
Kinjo, Masataka Browse this author →KAKEN DB
Ohta, Nobuhiro Browse this author →KAKEN DB
Keywords: FAD
autofluorescence
living cell
fluorescence lifetime imaging
intracellular pH
fluorescence decay
Issue Date: Jan-2013
Publisher: MDPI
Journal Title: International Journal of Molecular Sciences
Volume: 14
Issue: 1
Start Page: 1952
End Page: 1963
Publisher DOI: 10.3390/ijms14011952
Abstract: We have studied physiological parameters in a living cell using fluorescence lifetime imaging of endogenous chromophores. In this study, pH dependence of the fluorescence lifetime of flavin adenine dinucleotide (FAD), that is a significant cofactor exhibiting autofluorescence, has been investigated in buffer solution and in cells. The fluorescence lifetime of FAD remained unchanged with pH 5 to 9 in solution. However, the fluorescence lifetime in HeLa cells was found to decrease with increasing intracellular pH, suggesting that pH in a single cell can be estimated from the fluorescence lifetime imaging of FAD without adding exogenous fluorescent probes.
Rights: http://creativecommons.org/licenses/by/3.0/
Type: article
URI: http://hdl.handle.net/2115/52120
Appears in Collections:電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 太田 信廣

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