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Enzymatic properties and primary structures of two α-amylase isozymes from the Pacific abalone Haliotis discus hannai

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Title: Enzymatic properties and primary structures of two α-amylase isozymes from the Pacific abalone Haliotis discus hannai
Authors: Kumagai, Yuya Browse this author
Satoh, Takuya Browse this author
Inoue, Akira Browse this author →KAKEN DB
Ojima, Takao Browse this author →KAKEN DB
Keywords: Abalone
Haliotis
α-Amylase
cDNA cloning
GHF-13
Issue Date: Feb-2013
Publisher: Elsevier
Journal Title: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
Volume: 164
Issue: 2
Start Page: 80
End Page: 88
Publisher DOI: 10.1016/j.cbpb.2012.11.001
PMID: 23142215
Abstract: Two α-amylase (EC 3.2.1.1) isozymes, HdAmy58 and HdAmy82, with approximate molecular masses of 58 kDa and 82 kDa, respectively, were isolated from the digestive fluid of the Pacific abalone Haliotis discus hannai. Optimal temperatures and pHs for HdAmy58 and HdAmy82 were at 30℃ and 6.7, and 30℃ and 6.1, respectively. Both enzymes similarly degraded starch, glycogen, and maltooligosaccharides larger than maltotriose producing maltose and maltotriose as the major degradation products. However, the activity toward maltotetraose was appreciably higher in HdAmy82 than HdAmy58. cDNAs encoding HdAmy58 and HdAmy82 were cloned and the amino-acid sequences of 511 and 694 residues for HdAmy58 and HdAmy82, respectively, were deduced. The putative catalytic domains of HdAmy58 and HdAmy82 were located in the 17-511th and 19-500th amino-acid regions, respectively, and they showed approximately 50% amino-acid identity to each other. These sequences also showed 62-99% amino-acid identity to the catalytic domains of known α-amylases that belong to glycoside-hydrolase-family 13. The difference in the molecular masses between HdAmy58 and HdAmy82 was ascribed to the extension of approximately 190 residues in the C-terminus of HdAmy82. This extended region showed 41-63% amino-acid identity with the ancillary domains of several α-amylases previously reported.
Type: article (author version)
URI: http://hdl.handle.net/2115/52129
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 尾島 孝男

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