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Both antiviral activity and intracellular localization of chicken Mx protein depend on a polymorphism at amino acid position 631

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Title: Both antiviral activity and intracellular localization of chicken Mx protein depend on a polymorphism at amino acid position 631
Authors: Sasaki, Keisuke Browse this author
Yoneda, Akihiro Browse this author →KAKEN DB
Ninomiya, Akinori Browse this author
Kawahara, Manabu Browse this author →KAKEN DB
Watanabe, Tomomasa Browse this author →KAKEN DB
Keywords: Mx
Chicken
Polymorphism
Vesicular stomatitis virus
Intracellular localization
Issue Date: 4-Jan-2013
Publisher: Elsevier
Journal Title: Biochemical and Biophysical Research Communications
Volume: 430
Issue: 1
Start Page: 161
End Page: 166
Publisher DOI: 10.1016/j.bbrc.2012.11.053
PMID: 23201406
Abstract: The Mx protein is known to inhibit the multiplication of several RNA viruses. In chickens, a polymorphism at amino acid position 631 (631 aa) of Mx protein has been suggested to be involved in the antiviral ability against vesicular stomatitis virus (VSV) and influenza virus, indicating that a Ser-to-Asn substitution at 631 aa is the source of this antiviral ability. However, how the substitution at 631 aa contributes to the antiviral activity remains to be clarified. In this study, we investigated differences in antiviral activity against VSV and intracellular localization between Ser and Asn types at 631 aa of the chicken Mx protein. The results showed that chicken Mx protein with an Asn at 631 aa inhibited VSV multiplication and Mx distribution in a granular-like pattern in the cytoplasm. However, Mx carrying the Ser type did not inhibit viral growth and homogenous spread throughout the cytoplasm. Furthermore, we found that replacing Ser with Asn at 631 aa provided Mx with antiviral activity against VSV, with Mx showing granular-like distribution in the cytoplasm. These results demonstrated that a single amino acid polymorphism at 631 aa of the chicken Mx protein altered both the antiviral activity and intracellular localization.
Type: article (author version)
URI: http://hdl.handle.net/2115/52138
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 川原 学

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