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Expression of vascular endothelial growth factor C in human pterygium

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Title: Expression of vascular endothelial growth factor C in human pterygium
Authors: Fukuhara, Junichi Browse this author
Kase, Satoru Browse this author →KAKEN DB
Ohashi, Tsutomu Browse this author
Ando, Ryo Browse this author
Dong, Zhenyu Browse this author
Noda, Kousuke Browse this author →KAKEN DB
Ohguchi, Takeshi Browse this author
Kanda, Atsuhiro Browse this author
Ishida, Susumu Browse this author →KAKEN DB
Keywords: VEGF-C
VEGFR-3
Lymphangiogenesis
Lymphatic vessel density
Pterygium
Issue Date: Feb-2013
Publisher: Springer-Verlag
Journal Title: Histochemistry and Cell Biology
Volume: 139
Issue: 2
Start Page: 381
End Page: 389
Publisher DOI: 10.1007/s00418-012-1019-z
PMID: 22910845
Abstract: Purpose: Vascular endothelial growth factor C (VEGF-C) and its receptor VEGFR-3 mediate lymphangiogenesis. In this study, we analyzed the expression of VEGF-C and VEGFR-3 as well as lymphatic vessels in the pterygium and normal conjunctiva of humans. Methods: Fifteen primary nasal pterygia and three normal bulbar conjunctivas, surgically removed, were examined in this study. The lymphatic vessel density (LVD) and blood vessel density (BVD) were determined by the immunolabeling of D2-40 and CD31, markers for lymphatic and blood vessels, respectively. VEGF-C and VEGFR-3 expression in pterygial and conjunctival tissue proteins was detected by Western blotting. Expressions of VEGF-C and VEGFR-3 were evaluated using immunohistochemistry. Results: The LVD was significantly higher in the pterygium than normal conjunctiva (p<0.05). Western blot demonstrated high-level expression of VEGF-C and VEGFR-3 in the pterygium compared with normal conjunctiva. VEGF-C immunoreactivity was detected in the cytoplasm of pterygial and normal conjunctival epithelial cells. The number of VEGF-C-immunopositive cells in pterygial epithelial cells was significantly higher than in normal conjunctival cells (p<0.05). VEGFR-3 immunoreactivity was localized in the D2-40-positive lymphatic endothelial cells. Conclusions: The present findings suggest the potential role of VEGF-C in the pathogenesis and development of a pterygium through lymphangiogenesis and the VEGF-C/VEGFR-3 pathway as a novel therapeutic target for the human pterygium.
Rights: The final publication is available at www.springerlink.com
Type: article (author version)
URI: http://hdl.handle.net/2115/52139
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 加瀬 諭

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