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Visualizing hippocampal neurons with in vivo two-photon microscopy using a 1030 nm picosecond pulse laser

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Title: Visualizing hippocampal neurons with in vivo two-photon microscopy using a 1030 nm picosecond pulse laser
Authors: Kawakami, Ryosuke Browse this author →KAKEN DB
Sawada, Kazuaki Browse this author
Sato, Aya Browse this author
Hibi, Terumasa Browse this author →KAKEN DB
Kozawa, Yuichi Browse this author
Sato, Shunichi Browse this author
Yokoyama, Hiroyuki Browse this author
Nemoto, Tomomi Browse this author →KAKEN DB
Keywords: Fluorescence imaging
Multiphoton microscopy
Diode lasers
Issue Date: 24-Jan-2013
Publisher: Nature Publishing Group
Journal Title: Scientific Reports
Volume: 3
Start Page: 1014
Publisher DOI: 10.1038/srep01014
Abstract: In vivo two-photon microscopy has revealed vital information on neural activity for brain function, even in light of its limitation in imaging events at depths greater than several hundred micrometers from the brain surface. We developed a novel semiconductor-laser-based light source with a wavelength of 1030 nm that can generate pulses of 5-picosecond duration with 2-W output power, and a 20-MHz repetition rate. We also developed a system to secure the head of the mouse under an upright microscope stage that has a horizontal adjustment mechanism. We examined the penetration depth while imaging the H-Line mouse brain and demonstrated that our newly developed laser successfully images not only cortex pyramidal neurons spreading to all cortex layers at a superior signal-to-background ratio, but also images hippocampal CA1 neurons in a young adult mouse.
Type: article
Appears in Collections:電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 根本 知己

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