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Enhanced propagation of fish nodaviruses in BF-2 cells persitently infected with snakehead retrovirus (SnRV)

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Title: Enhanced propagation of fish nodaviruses in BF-2 cells persitently infected with snakehead retrovirus (SnRV)
Authors: Nishizawa, Toyohiko Browse this author →KAKEN DB
Kokawa, Yuki Browse this author
Wakayama, Takao Browse this author
Kinoshita, Shinichi Browse this author
Yoshimizu, Mamoru Browse this author →KAKEN DB
Keywords: Persistent infection
Fish nodavirus
Issue Date: 3-Mar-2008
Publisher: Inter-Research
Journal Title: Diseases of Aquatic Organisms
Volume: 79
Issue: 1
Start Page: 19
End Page: 25
Publisher DOI: 10.3354/dao01882
PMID: 18429438
Abstract: Fish nodaviruses are causative agents of viral nervous necrosis causing high mortality in cultured marine fishes around the world. The first successful isolation of fish nodavirus was made with SSN-1 cells, which are persistently infected with snakehead retrovirus (SnRV). In the present study, a BF-2 cell line persistently infected with SnRV (PI-BF-2) was established to evaluate the influence of SnRV on the production of fish nodavirus. The PI-BF-2 cells were slightly more slender than BF-2 cells, but no difference was observed in propagation rate between both cell lines. No difference was observed in production of SnRV between PI-BF-2 and SSN-1 cell lines. Although both PI-BF-2 and BF-2 cell lines showed no cytopathic effect (CPE) after inoculation of striped jack nervous necrosis virus (SJNNV) and redspotted grouper nervous necrosis virus (RGNNV), these fish nodaviruses could be amplified in BF-2 cells, and moreover, production of fish nodaviruses in the PI-BF-2 cell line was more than 40 times higher than in BF-2 cells. Thus, it was concluded that BF-2 cell permissiveness to fish nodaviruses was enhanced by persistent infection with SnRV. Furthermore, homologous cDNA to genomic RNA of SJNNV was detected from both PI-BF-2 and SSN-1 cell lines persistently infected with SnRV. The amount of nodavirus cDNA in SJNNV-inoculated PI-BF-2 cells was clearly lower than that in SJNNV-inoculated SSN-1 cells.
Rights: © 2008 Inter-Research
Type: article
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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