HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Health Sciences / Faculty of Health Sciences >
Peer-reviewed Journal Articles, etc >

Chlamydophila pneumoniae in human immortal Jurkat cells and primary lymphocytes uncontrolled by interferon-γ

Files in This Item:
yamaguchi_Chlamydophila0130.pdf1.51 MBPDFView/Open
Please use this identifier to cite or link to this item:

Title: Chlamydophila pneumoniae in human immortal Jurkat cells and primary lymphocytes uncontrolled by interferon-γ
Authors: Ishida, Kasumi Browse this author
Kubo, Takeru Browse this author
Saeki, Ayumi Browse this author →KAKEN DB
Yamane, Chikayo Browse this author
Matsuo, Junji Browse this author →KAKEN DB
Yimin Browse this author →KAKEN DB
Nakamura, Shinji Browse this author →KAKEN DB
Hayashi, Yasuhiro Browse this author →KAKEN DB
Kunichika, Miyuki Browse this author
Yoshida, Mitsutaka Browse this author
Takahashi, Kaori Browse this author
Hirai, Itaru Browse this author
Yamamoto, Yoshimasa Browse this author
Shibata, Ken-Ichiro Browse this author →KAKEN DB
Yamaguchi, Hiroyuki Browse this author →KAKEN DB
Keywords: Chlamydophila pneumoniae
Indoleamine 2,3-dioxygenase
Issue Date: Mar-2013
Journal Title: Microbes and infection / Institut Pasteur
Volume: 15
Issue: 3
Start Page: 192
End Page: 200
Publisher DOI: 10.1016/j.micinf.2012.11.006
PMID: 23178757
Abstract: Lymphocytes are a potential host cell for Chlamydophila pneumoniae, although why the bacteria must hide in lymphocytes remains unknown. Meanwhile, interferon (IFN)-γ is a crucial factor for eliminating chlamydiae from infected cells through indoleamine 2,3-dioxygenase (IDO) expression, resulting in depletion of tryptophan. We therefore assessed if lymphocytes could work as a shelter for the bacteria to escape IFN-γ. C. pneumoniae grew normally in human lymphoid Jurkat cells, even in the presence of IFN-γ or under stimulation with phorbol myristate acetate plus ionomycin. Although Jurkat cells expressed IFN-γ receptor CD119, their lack of IDO expression was confirmed by RT-PCR and western blotting. Also, C. pneumoniae survived in enriched human peripheral blood lymphocytes, even in the presence of IFN-γ. Furthermore, C. pneumoniae in spleen cells obtained from IFN-γ knockout mice with C57BL/6 background was maintained in a similar way to wild-type mice, supporting a minimal role of IFN-γ-related response for eliminating C. pneumoniae from lymphocytes. Thus, we concluded that IFN-γ did not remove C. pneumoniae from lymphocytes, possibly providing a shelter for C. pneumoniae to escape from the innate immune response, which has direct clinical significance.
Type: article (author version)
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山口 博之

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


Feedback - Hokkaido University