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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Fluorescence polarization-based assay using N-glycan-conjugated quantum dots for screening in hemagglutinin blockers for influenza A viruses
| Title: | Fluorescence polarization-based assay using N-glycan-conjugated quantum dots for screening in hemagglutinin blockers for influenza A viruses |
| Authors: | Okamatsu, Masatoshi Browse this author →KAKEN DB | | Feng, Fei Browse this author | | Ohyanagi, Tatsuya Browse this author | | Nagahori, Noriko Browse this author →KAKEN DB | | Someya, Kazuhiko Browse this author | | Sakoda, Yoshihiro Browse this author →KAKEN DB | | Miura, Nobuaki Browse this author →KAKEN DB | | Nishimura, Shin-Ichiro Browse this author →KAKEN DB | | Kida, Hiroshi Browse this author →KAKEN DB |
| Keywords: | Influenza virus | | Hemagglutinin | | Fluorescence polarization | | Quantum dot | | High-throughput screening |
| Issue Date: | Feb-2013 |
| Publisher: | Elsevier B.V. |
| Journal Title: | Journal of Virological Methods |
| Volume: | 187 |
| Issue: | 2 |
| Start Page: | 390 |
| End Page: | 394 |
| Publisher DOI: | 10.1016/j.jviromet.2012.11.004 |
| PMID: | 23164994 |
| Abstract: | Attachment of influenza virus to susceptible cells is mediated by viral protein hemagglutinin (HA), which recognizes cell surface glycoconjugates that terminate in α-sialosides. To develop anti-influenza drugs based on inhibition of HA-mediated infection, novel fluorescent nanoparticles displaying multiple biantennary N-glycan chains with α-sialosides (A2-PC-QDs) that have high affinity for the HA were designed and constructed. The A2-PC-QDs enabled an easy and efficient fluorescence polarization (FP) assay for detection of interaction with the HA and competitive inhibition even by small molecule compounds against A2-PC-QDs-HA binding. The quantum dot (QD)-based FP assay established in the present study is a useful tool for high-throughput screening and to accelerate the development of novel and more effective blockers of the viral attachment of influenza virus. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/52274 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 岡松 正敏
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