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Fluorescence polarization-based assay using N-glycan-conjugated quantum dots for screening in hemagglutinin blockers for influenza A viruses

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/52274

Title: Fluorescence polarization-based assay using N-glycan-conjugated quantum dots for screening in hemagglutinin blockers for influenza A viruses
Authors: Okamatsu, Masatoshi Browse this author →KAKEN DB
Feng, Fei Browse this author
Ohyanagi, Tatsuya Browse this author
Nagahori, Noriko Browse this author →KAKEN DB
Someya, Kazuhiko Browse this author
Sakoda, Yoshihiro Browse this author →KAKEN DB
Miura, Nobuaki Browse this author →KAKEN DB
Nishimura, Shin-Ichiro Browse this author →KAKEN DB
Kida, Hiroshi Browse this author →KAKEN DB
Keywords: Influenza virus
Hemagglutinin
Fluorescence polarization
Quantum dot
High-throughput screening
Issue Date: Feb-2013
Publisher: Elsevier B.V.
Journal Title: Journal of Virological Methods
Volume: 187
Issue: 2
Start Page: 390
End Page: 394
Publisher DOI: 10.1016/j.jviromet.2012.11.004
PMID: 23164994
Abstract: Attachment of influenza virus to susceptible cells is mediated by viral protein hemagglutinin (HA), which recognizes cell surface glycoconjugates that terminate in α-sialosides. To develop anti-influenza drugs based on inhibition of HA-mediated infection, novel fluorescent nanoparticles displaying multiple biantennary N-glycan chains with α-sialosides (A2-PC-QDs) that have high affinity for the HA were designed and constructed. The A2-PC-QDs enabled an easy and efficient fluorescence polarization (FP) assay for detection of interaction with the HA and competitive inhibition even by small molecule compounds against A2-PC-QDs-HA binding. The quantum dot (QD)-based FP assay established in the present study is a useful tool for high-throughput screening and to accelerate the development of novel and more effective blockers of the viral attachment of influenza virus.
Type: article (author version)
URI: http://hdl.handle.net/2115/52274
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 岡松 正敏

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