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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >
Irradiation-tolerant lung cancer cells acquire invasive ability dependent on dephosphorylation of the myosin regulatory light chain
| Title: | Irradiation-tolerant lung cancer cells acquire invasive ability dependent on dephosphorylation of the myosin regulatory light chain |
| Authors: | Ishihara, Seiichiro Browse this author | | Yasuda, Motoaki Browse this author | | Nishioka, Takeshi Browse this author | | Mizutani, Takeomi Browse this author | | Kawabata, Kazushige Browse this author | | Shirato, Hiroki Browse this author | | Haga, Hisashi Browse this author →KAKEN DB |
| Keywords: | 3D culture | | Collagen gel | | Irradiation | | Invasion | | Lung adenocarcinoma | | Myosin regulatory light chain |
| Issue Date: | 18-Mar-2013 |
| Publisher: | Elsevier |
| Journal Title: | FEBS Letters |
| Volume: | 587 |
| Issue: | 6 |
| Start Page: | 732 |
| End Page: | 736 |
| Publisher DOI: | 10.1016/j.febslet.2013.01.055 |
| PMID: | 23391761 |
| Abstract: | Radiotherapy is one of the major treatment modalities for malignancies. However, cells surviving irradiation often display high levels of invasiveness. This study shows that irradiation-tolerant lung adenocarcinoma demonstrates high invasive capability depending on dephosphorylation of the myosin regulatory light chain (MRLC). In a collagen gel overlay condition, low-invasive subclones of lung adenocarcinoma (A549P-3) showed a round morphology and diphosphorylation of MRLC. In contrast, irradiation-tolerant A549P-3 cells (A549P-3IR) displayed high invasiveness and a lower level of MRLC diphosphorylation. In addition, inhibition of MRLC phosphatase activity decreased the invasive activity. These findings suggest that A549P-3IR cells acquire high invasiveness through MRLC dephosphorylation. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved. |
| Relation: | http://www.sciencedirect.com/science/article/pii/S0014579313001002 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/52653 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 芳賀 永
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