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Irradiation-tolerant lung cancer cells acquire invasive ability dependent on dephosphorylation of the myosin regulatory light chain

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Title: Irradiation-tolerant lung cancer cells acquire invasive ability dependent on dephosphorylation of the myosin regulatory light chain
Authors: Ishihara, Seiichiro Browse this author
Yasuda, Motoaki Browse this author
Nishioka, Takeshi Browse this author
Mizutani, Takeomi Browse this author
Kawabata, Kazushige Browse this author
Shirato, Hiroki Browse this author
Haga, Hisashi Browse this author →KAKEN DB
Keywords: 3D culture
Collagen gel
Irradiation
Invasion
Lung adenocarcinoma
Myosin regulatory light chain
Issue Date: 18-Mar-2013
Publisher: Elsevier
Journal Title: FEBS Letters
Volume: 587
Issue: 6
Start Page: 732
End Page: 736
Publisher DOI: 10.1016/j.febslet.2013.01.055
PMID: 23391761
Abstract: Radiotherapy is one of the major treatment modalities for malignancies. However, cells surviving irradiation often display high levels of invasiveness. This study shows that irradiation-tolerant lung adenocarcinoma demonstrates high invasive capability depending on dephosphorylation of the myosin regulatory light chain (MRLC). In a collagen gel overlay condition, low-invasive subclones of lung adenocarcinoma (A549P-3) showed a round morphology and diphosphorylation of MRLC. In contrast, irradiation-tolerant A549P-3 cells (A549P-3IR) displayed high invasiveness and a lower level of MRLC diphosphorylation. In addition, inhibition of MRLC phosphatase activity decreased the invasive activity. These findings suggest that A549P-3IR cells acquire high invasiveness through MRLC dephosphorylation. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
Relation: http://www.sciencedirect.com/science/article/pii/S0014579313001002
Type: article (author version)
URI: http://hdl.handle.net/2115/52653
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 芳賀 永

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