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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
A horizontally transferred tRNACys gene in the sugar beet mitochondrial genome: evidence that the gene is present in diverse angiosperms and its transcript is aminoacylated
| Title: | A horizontally transferred tRNACys gene in the sugar beet mitochondrial genome: evidence that the gene is present in diverse angiosperms and its transcript is aminoacylated |
| Authors: | Kitazaki, Kazuyoshi Browse this author | | Kubo, Tomohiko Browse this author →KAKEN DB | | Kagami, Hiroyo Browse this author | | Matsumoto, Takuma Browse this author | | Fujita, Asami Browse this author | | Matsuhira, Hiroaki Browse this author | | Matsunaga, Muneyuki Browse this author | | Mikami, Tetsuo Browse this author →KAKEN DB |
| Keywords: | horizontal gene transfer | | plant mitochondria | | angiosperm | | tRNA | | dual targeting | | aminoacyl-tRNA synthetase |
| Issue Date: | Oct-2011 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | The Plant Journal |
| Volume: | 68 |
| Issue: | 2 |
| Start Page: | 262 |
| End Page: | 272 |
| Publisher DOI: | 10.1111/j.1365-313X.2011.04684.x |
| PMID: | 21699590 |
| Abstract: | Of the two tRNACys(GCA) genes, trnC1-GCA and trnC2-GCA, previously identified in mitochondrial genome of sugar beet, the former is a native gene and probably a pseudo copy, whereas the latter, of origin unknown, is transcribed into a tRNA [tRNACys2(GCA)]. In this study, the trnC2-GCA sequence was mined from various public databases. To evaluate whether or not the trnC2-GCA sequence is located in the mitochondrial genome, the relative copy number of its sequence was assessed in a number of angiosperm species, using a quantitative real time PCR assay. The trnC2-GCA sequence was found to exist sporadically in the mitochondrial genomes of a wide range of angiosperms. The mitochondrial tRNACys2(GCA) species from sugar beet, spinach and cucumber were found to be aminoacylated, indicating that they may participate in translation. We also identified a sugar beet nuclear gene that encodes cysteinyl-tRNA synthetase, which is dual-targeted to mitochondria and plastids, and may aminoacylate tRNACys2(GCA). What is of particular interest is that trnC1-GCA and trnC2-GCA co-exist in the mitochondrial genomes of eight diverse angiosperms, including spinach, and that the spinach tRNACys1(GCA) is also aminoacylated. Taken together, our observations lead us to surmise that trnC2-GCA might have been horizontally transferred to a common ancestor of eudicots, followed by co-existence and dual-expression of trnC1-GCA and trnC2-GCA in mitochondria with occasional loss or inactivation of either trnC-GCA gene during evolution. |
| Rights: | The definitive version is available at www.blackwell-synergy.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/52685 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 久保 友彦
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