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Inhibition of Marburg Virus Budding by Nonneutralizing Antibodies to the Envelope Glycoprotein
| Title: | Inhibition of Marburg Virus Budding by Nonneutralizing Antibodies to the Envelope Glycoprotein |
| Authors: | Kajihara, Masahiro Browse this author | | Marzi, Andrea Browse this author | | Nakayama, Eri Browse this author | | Noda, Takeshi Browse this author | | Kuroda, Makoto Browse this author | | Manzoor, Rashid Browse this author | | Matsuno, Keita Browse this author | | Feldmann, Heinz Browse this author | | Yoshida, Reiko Browse this author | | Kawaoka, Yoshihiro Browse this author | | Takada, Ayato Browse this author →KAKEN DB |
| Issue Date: | Dec-2012 |
| Publisher: | American Society for Microbiology |
| Journal Title: | Journal of Virology |
| Volume: | 86 |
| Issue: | 24 |
| Start Page: | 13467 |
| End Page: | 13474 |
| Publisher DOI: | 10.1128/JVI.01896-12 |
| Abstract: | The envelope glycoprotein (GP) of Marburg virus (MARV) and Ebola virus (EBOV) is responsible for virus entry into host cells and known as the only target of neutralizing antibodies. While knowledge about EBOV neutralizing antibodies and the mechanism for neutralization of infectivity is being accumulated gradually, little is known about antibodies that can efficiently regulate MARV infectivity. Here we show that MARV GP-specific monoclonal antibodies AGP127-8 (IgG1) and MGP72-17 (IgM), which do not inhibit the GP-mediated entry of MARV into host cells, drastically reduced budding and release of progeny viruses from infected cells. These antibodies similarly inhibited the formation of virus-like particles (VLPs) consisting of GP, the viral matrix protein, and nucleoprotein, whereas the Fab fragment of AGP127-8 showed no inhibitory effect. Morphological analyses revealed that filamentous VLPs were bunched on the surface of VLP-producing cells cultured in the presence of the antibodies. These results demonstrate a novel mechanism of the antibody-mediated inhibition of MARV budding in which antibodies arrest unformed virus particles on the cell surface. Our data lead to the idea that such antibodies, like classical neutralizing antibodies, contribute to protective immunity against MARV and that the "classical" neutralizing activity is not the only indicator of a protective antibody that may be available for prophylactic and therapeutic use. |
| Rights: | © 2012 American Society for Microbiology |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/52795 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 高田 礼人
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