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Three types of neurochemical projection from the bed nucleus of the stria terminalis to the ventral tegmental area in adult mice.

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Title: Three types of neurochemical projection from the bed nucleus of the stria terminalis to the ventral tegmental area in adult mice.
Authors: Kudo, Takehiro Browse this author
Uchigashima, Motokazu Browse this author →KAKEN DB
Miyazaki, Taisuke Browse this author →KAKEN DB
Konno, Kohtarou Browse this author
Yamasaki, Miwako Browse this author →KAKEN DB
Yanagawa, Yuchio Browse this author
Minami, Masabumi Browse this author →KAKEN DB
Watanabe, Masahiko Browse this author →KAKEN DB
Issue Date: 12-Dec-2012
Publisher: Society for Neuroscience
Journal Title: The Journal of neuroscience : the official journal of the Society for Neuroscience
Volume: 32
Issue: 50
Start Page: 18035
End Page: 18046
Publisher DOI: 10.1523/JNEUROSCI.4057-12.2012
PMID: 23238719
Abstract: Dopaminergic (DAergic) neurons in the ventral tegmental area (VTA) play crucial roles in motivational control of behaviors, and their activity is regulated directly or indirectly via GABAergic neurons by extrinsic afferents from various sources, including the bed nucleus of the stria terminalis (BST). Here, the neurochemical composition of VTA-projecting BST neurons and their outputs to the VTA were studied in adult mouse brains. By combining retrograde tracing with fluorescence in situ hybridization for 67 kDa glutamate decarboxylase (GAD67) and vesicular glutamate transporters (VGluTs), VTA-targeting BST neurons were classified into GAD67-positive (GAD67(+))/VGluT3-negative (VGluT3(-)), GAD67(+)/VGluT3(+), and VGluT2(+) neurons, of which GAD67(+)/VGluT3(-) neurons constituted the majority (∼90%) of VTA-projecting BST neurons. GABAergic efferents from the BST formed symmetrical synapses on VTA neurons, which were mostly GABAergic neurons, and expressed GABA(A) receptor α1 subunit on their synaptic and extrasynaptic membranes. In the VTA, VGluT3 was detected in terminals expressing vesicular inhibitory amino acid transporter (VIAAT), plasmalemmal serotonin transporter, or neither. Of these, VIAAT(+)/VGluT3(+) terminals, which should include those from GAD67(+)/VGluT3(+) BST neurons, formed symmetrical synapses. When single axons from VGluT3(+) BST neurons were examined, almost all terminals were labeled for VIAAT, whereas VGluT3 was often absent from terminals with high VIAAT loads. VGluT2(+) terminals in the VTA exclusively formed asymmetrical synapses, which expressed AMPA receptors on postsynaptic membrane. Therefore, the major mode of the BST-VTA projection is GABAergic, and its activation is predicted to disinhibit VTA DAergic neurons. VGluT2(+) and VGluT3(+) BST neurons further supply additional projections, which may principally convey excitatory or inhibitory inputs, respectively, to the VTA.
Relation: http://www.jneurosci.org/content/32/50/18035.full
Type: article
URI: http://hdl.handle.net/2115/52919
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡邉 雅彦

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