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Immunolocalization of sclerostin synthesized by osteocytes in relation to bone remodeling in the interradicular septa of ovariectomized rats

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Title: Immunolocalization of sclerostin synthesized by osteocytes in relation to bone remodeling in the interradicular septa of ovariectomized rats
Authors: Guo, Ying Browse this author
Li, Minqi Browse this author →KAKEN DB
Liu, Zhusheng Browse this author
Yamada, Tamaki Browse this author
Sasaki, Muneteru Browse this author
Hasegawa, Tomoka Browse this author
Hongo, Hiromi Browse this author
Tabata, Chihiro Browse this author
Suzuki, Reiko Browse this author →KAKEN DB
Oda, Kimimitsu Browse this author
Yamamoto, Tsuneyuki Browse this author →KAKEN DB
Kawanami, Masamitsu Browse this author →KAKEN DB
Amizuka, Norio Browse this author →KAKEN DB
Keywords: ovariectomy
estrogen receptor α
alveolar bone
Issue Date: Oct-2012
Publisher: Oxford University Press
Journal Title: Journal of Electron Microscopy
Volume: 61
Issue: 5
Start Page: 309
End Page: 320
Publisher DOI: 10.1093/jmicro/dfs052
PMID: 22802488
Abstract: This study aimed to elucidate whether estrogen deficiency would affect the synthesis of an osteocyte-derived factor, sclerostin, in the mesial region of alveolar bone. Eight 9-week-old Wistar female rats were ovariectomized (OVX) and the other eight rats were Sham-operated (Sham). After 4 weeks, the interradicular septa of mandibular first molar were embedded in paraffin, and then, were histochemically examined. Sclerostin-positive osteocytes were located in a superficial layer of the mesial region of Sham bone, while the OVX mesial region showed a lesser presence of the sclerostin-reactive osteocytes. There was no significant difference in the distribution of estrogen receptor α and TUNEL-positive cells in either Sham or OVX groups. Meanwhile, the Sham mesial region demonstrated many osteoclasts, but the OVX specimens showed numerous osteoclasts in association with intense immunolabeling of the receptor activator of the nuclear factor kB ligand. Complex meshwork of cement lines was seen consistent with irregularly-distributed osteocytic lacunar-canalicular system in the OVX mesial region, compared with those of the Sham specimens. In conclusion, estrogen deficiency appears to inhibit osteocytes for sclerostin synthesis in the mesial region of the interradicular septum, mediated by accelerated bone remodeling, rather than by directly effecting osteocytes.
Rights: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Electron Microscopy following peer review. The definitive publisher-authenticated version J Electron Microsc (Tokyo) (2012) 61 (5): 309-320 is available online at:
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 網塚 憲生

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