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Production of androgenetic diploid loach by cold-shock of eggs fertilized with diploid sperm

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Title: Production of androgenetic diploid loach by cold-shock of eggs fertilized with diploid sperm
Authors: Hou, Jilun Browse this author
Fujimoto, Takafumi Browse this author
Yamaha, Etsuro Browse this author →KAKEN DB
Arai, Katsutoshi Browse this author →KAKEN DB
Keywords: Cold-shock
Misgurnus anguillicaudatus
Issue Date: 15-Jul-2013
Publisher: Elsevier
Journal Title: Theriogenology
Volume: 80
Issue: 2
Start Page: 125
End Page: 130
Publisher DOI: 10.1016/j.theriogenology.2013.03.014
PMID: 23602217
Abstract: Diploid androgenotes were produced without egg irradiation in the loach, Misgurnus anguillicaudatus. Eggs of wild-type diploid females were fertilized with diploid sperm of a neo-tetraploid male and then cold-shock treated at 3 °C (range, ±0.5 °C) for 30 minutes just after fertilization to eliminate the female nucleus. After hatching, ploidy status of the hatched larvae was analyzed by flow cytometry, which revealed putative diploid androgenotes as well as larvae possessing other ploidies. Five independent microsatellite DNA markers were genotyped to confirm all-male inheritance of the resultant diploid larvae. The mean ± SD yield rate of diploid androgenetic larvae to total eggs used was 12.29 ± 3.25% in the cold-shock group and 22.23 ± 13.42% in the UV-irradiated group (P > 0.05). No diploid androgenetic larvae were detected in the intact control group. To our knowledge, this is the first report demonstrating successful induction of diploid androgenotes without egg irradiation in fish.
Type: article (author version)
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 荒井 克俊

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