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Improvement of Enzymatic Saccharification of Unbleached Cedar Pulp with Amphipathic Lignin Derivatives

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Title: Improvement of Enzymatic Saccharification of Unbleached Cedar Pulp with Amphipathic Lignin Derivatives
Authors: Winarni, Ina Browse this author
Oikawa, Chihiro Browse this author
Yamada, Tatsuhiko Browse this author
Igarashi, Kiyohiko Browse this author
Koda, Keiichi Browse this author
Uraki, Yasumitsu Browse this author →KAKEN DB
Keywords: Amphipathic lignin derivatives
Enzymatic saccharification
Recovery of cellulase activity
Issue Date: 2013
Publisher: North Carolina State University
Journal Title: Bioresources
Volume: 8
Issue: 2
Start Page: 2195
End Page: 2208
Abstract: Synthesized amphipathic lignin derivatives comprised of acidic acid lignin (AL) with poly(ethylene glycol) diglycidyl ether (PEGDE), ethoxy-(2-hydroxy)-propoxy-poly(ethylene glycol) glycidyl ether (EPEG) or dodecyloxy-poly(ethylene glycol) glycidyl ether (DAEO) were added before the enzymatic saccharification of unbleached cedar pulp along with two commercially available cellulases, Meicelase and Genencor GC220. At the same filter paper unit (FPU) dosage, GC220 showed higher sugar yield than Meicelase. The difference was attributed to the composition of processive and non-processive endoglucanase activities per FPU; GC220 had higher such activities than Meicelase. The sugar yield was significantly improved by the addition of the lignin derivatives. In addition, residual activities after the saccharification were maintained at the higher level by their addition than with polyethylene glycol (PEG) 4000. In particular, EPEG-AL yielded the complete recovery of cellulase activity when using 20 FPU/g of substrate. It was found that the lignin derivatives were directly associated with Cel6A, one of cellulase components, whereas PEG 4000 was not. Thus, it is evident that the lignin derivatives are promising agents to improve the enzymatic saccharification of cellulase.
Type: article
URI: http://hdl.handle.net/2115/53082
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 浦木 康光

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