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Characterization of Meq proteins from field isolates of Marek's disease virus in Japan

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Title: Characterization of Meq proteins from field isolates of Marek's disease virus in Japan
Authors: Murata, Shiro Browse this author →KAKEN DB
Hashiguchi, Tomoyuki Browse this author
Hayashi, Yuko Browse this author
Yamamoto, Yuki Browse this author
Matsuyama-Kato, Ayumi Browse this author
Takasaki, Sarah Browse this author
Isezaki, Masayoshi Browse this author
Onuma, Misao Browse this author →KAKEN DB
Konnai, Satoru Browse this author →KAKEN DB
Ohashi, Kazuhiko Browse this author →KAKEN DB
Keywords: Marek's disease virus
Distinct diversity
Transactivation activity
Issue Date: Jun-2013
Publisher: Elsevier Science Bv
Journal Title: Infection Genetics And Evolution
Volume: 16
Start Page: 137
End Page: 143
Publisher DOI: 10.1016/j.meegid.2012.12.032
PMID: 23352889
Abstract: Serotype 1 strains of Marek's disease virus (MDV-1) cause malignant lymphomas in chickens (Marek's disease; MD). Although MD has been controlled by vaccination, field isolates of MDV-1 have tended to increase in virulence and cause MD even in vaccinated chickens. Meq, a putative MDV-1 oncoprotein, resembles the Jun/Fos family of basic leucine zipper (bZIP) transcription factors and can regulate the expression of viral and cellular genes as a homodimer or as a heterodimer with a variety of bZIP family proteins. Sequencing analysis of some of the viral genes of various MDV-1 strains revealed a distinct diversity of and point mutations in Meq, which may contribute to changes in the transcriptional activities of Meq and, consequently, to increases in MDV-1 oncogenicity. However, few reports have characterized MDV-1 strains isolated in Japan. In this study, we established the amino acid sequences of MDV-1 field isolates from Japan in order to determine whether they display a distinct diversity of and point mutations in Meq. In addition, we analyzed the transactivation activities of the Meq proteins in order to evaluate whether the observed mutations affect their functions. Japanese MDV-1 isolates displayed the distinct mutations in basic region 2 (BR2) and proline-rich repeats (PRRs) of the Meq proteins as well as some unique mutations. Reporter assays revealed that the amino acid substitutions in BR2 and the PRRs affected the Meq transactivation activity. These results suggest that the distinct mutations are also present in the Meq proteins of MDV-1 isolates from Japan and affect their transactivation activities. (C) 2013 Elsevier B.V. All rights reserved.
Type: article (author version)
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 村田 史郎

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