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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
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Preliminary X-ray crystallographic study of staphylococcal alpha-haemolysin monomer
| Title: | Preliminary X-ray crystallographic study of staphylococcal alpha-haemolysin monomer |
| Authors: | Sugawara, Takaki Browse this author | | Yamashita, Daichi Browse this author | | Tanaka, Yoshikazu Browse this author →KAKEN DB | | Kaneko, Jun Browse this author →KAKEN DB | | Kamio, Yoshiyuki Browse this author →KAKEN DB | | Tanaka, Isao Browse this author →KAKEN DB | | Yao, Min Browse this author →KAKEN DB |
| Keywords: | staphylococcal | | alpha-haemolysin | | monomer | | pore-forming toxins |
| Issue Date: | Aug-2013 |
| Publisher: | International Union of Crystallography |
| Journal Title: | Acta Crystallographica Section F : Structural Biology and Crystallization Communications |
| Volume: | 69 |
| Issue: | 8 |
| Start Page: | 868 |
| End Page: | 870 |
| Publisher DOI: | 10.1107/S174430911301693X |
| Abstract: | Staphylococcal alpha-haemolysin is a beta-barrel pore-forming toxin expressed by Staphylococcus aureus. alpha-Haemolysin is secreted as a water-soluble monomeric protein which binds to target membranes and forms membrane-inserted heptameric pores. Although the crystal structures of the heptameric pore and monomer bound to an antibody have been determined, that of monomeric alpha-haemolysin without binder has yet to be elucidated. Previous mutation studies showed that mutants of His35 retain the monomeric structure but are unable to assemble into heptamers. Here, alpha-haemolysin H35A mutants were expressed, purified and crystallized. Diffraction data were collected to 2.90 angstrom resolution. The crystals belonged to space group P6(1), with unit-cell parameters a = b = 151.3, c = 145.0 angstrom. Molecular replacement found four molecules in an asymmetric unit. The relative orientation among molecules was distinct from that of the pore, indicating that the crystal contained monomeric alpha-haemolysin. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/53113 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 田中 良和
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