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Hokkaido University Collection of Scholarly and Academic Papers >
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Structure of dihydrouridine synthase C (DusC) from Escherichia coli
| Title: | Structure of dihydrouridine synthase C (DusC) from Escherichia coli |
| Authors: | Chen, Minghao Browse this author | | Yu, Jian Browse this author | | Tanaka, Yoshikazu Browse this author →KAKEN DB | | Tanaka, Miyuki Browse this author | | Tanaka, Isao Browse this author →KAKEN DB | | Yao, Min Browse this author →KAKEN DB |
| Keywords: | dihydrouridin | | dihydrouridine synthase | | tRNA modification |
| Issue Date: | Aug-2013 |
| Publisher: | International Union of Crystallography |
| Journal Title: | Acta Crystallographica Section F : Structural Biology And Crystallization Communications |
| Volume: | 69 |
| Issue: | 8 |
| Start Page: | 834 |
| End Page: | 838 |
| Publisher DOI: | 10.1107/S1744309113019489 |
| Abstract: | Dihydrouridine (D) is one of the most widely conserved tRNA modifications. Dihydrouridine synthase (Dus) is responsible for introducing D modifications into RNA by the reduction of uridine. Recently, a unique substrate-recognition mechanism using a small adapter molecule has been proposed for Thermus thermophilus Dus (TthDusC). To acquire insight regarding its substrate-recognition mechanism, the crystal structure of DusC from Escherichia coli (EcoDusC) was determined at 2.1 angstrom resolution. EcoDusC was shown to be composed of two domains: an N-terminal catalytic domain and a C-terminal tRNA-binding domain. An L-shaped electron density surrounded by highly conserved residues was found in the active site, as observed for TthDus. Structure comparison with TthDus indicated that the N-terminal region has a similar structure, whereas the C-terminal domain has marked differences in its relative orientation to the N-terminal domain as well as in its own structure. These observations suggested that Dus proteins adopt a common substrate-recognition mechanism using an adapter molecule, whereas the manner of tRNA binding is diverse. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/53114 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 田中 良和
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