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Title: サケ科魚類卵巣腔液からの伝染性造血器壊死症ウィルス(IHNV)の分離に適した細胞の選択
Other Titles: Selection of suitable cell line for isolation of the infectious hematopoietic necrosis virus (IHNV) from ovarian fluid of salmonid fish
Authors: 吉仲, 桃子1 Browse this author
吉水, 守2 Browse this author →KAKEN DB
絵面, 良男3 Browse this author →KAKEN DB
Authors(alt): Yoshinaka, Toko1
Yoshimizu, Mamoru2
Ezura, Yoshio3
Keywords: IHNV
Cell line
Cytotoxic effect
Issue Date: 15-Jun-1997
Publisher: 日本魚病学会
Journal Title: 魚病研究
Journal Title(alt): Fish pathology
Volume: 32
Issue: 2
Start Page: 75
End Page: 80
Publisher DOI: 10.3147/jsfp.32.75
Abstract: Replicability and yield of 2 strains of IHNV in 9 salmonid cell lines (CHSE-214, KO-6, RTE, RTG-2, RTH, RTT, SEH, STE, RTE-2) and 3 non-salmonid cell lines (FHM, EPC, EPG) were compared at a low M.O.I.of 0.001.Yield of IHNV in RTE-2 derived from rainbow trout embryo was as high as those of RTG-2, EPG, FHM and EPC cell lines. Although EPC and FHM cell lines showed high sensitivity to toxic substances in the ovarian fluid from masu salmon Oncorhychus masou, RTE-2 and RTG-2 showed low susceptibility to these substances.These results indicate that the RTE-2 and RTG-2 cell lines are suitable for the isolation of IHNV from the ovarian fluid.
IHNV の分離に適した細胞を選択する目的でサケ科魚類細胞9種とサケ科以外の魚類細胞3種を供試し, 2株のIHNV を接種して, 増殖速度と増殖量を比較した。ウイルスの増殖速度が速くかつ増殖量の多い細胞は RTE-2, RTG-2, EPG, FHM であった。EPC と FHM 細胞はサクラマス産卵腔液に含まれる細胞毒性成分に高い感受性を示した。RTG-2 および RTE-2 細胞は CPE を多く発現し, 細胞毒性発現は少数であった。以上より, 卵巣腔液中のIHNV 分離に適した細胞として RTE-2 および RTG-2 細胞を選択した。
Type: article
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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