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Detection of antibody against lymphocystis disease virus in Japanese flounder by enzyme linked immunosorbent assay

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Title: Detection of antibody against lymphocystis disease virus in Japanese flounder by enzyme linked immunosorbent assay
Other Titles: ELISAによるヒラメのリンホシスチス病ウイルスに対する抗体の検出
Authors: Nishida, Hiroko1 Browse this author
Yoshimizu, Mamoru2 Browse this author →KAKEN DB
Ezura, Yoshio3 Browse this author →KAKEN DB
Authors(alt): 西田, 弘子1
吉水, 守2
絵面, 良男3
Keywords: Lymphocystis disease
LCDV
Japanese flounder
Antibody
ELISA
Issue Date: 20-Oct-1998
Publisher: 日本魚病学会
Journal Title: 魚病研究
Journal Title(alt): Fish pathology
Volume: 33
Issue: 4
Start Page: 207
End Page: 211
Publisher DOI: 10.3147/jsfp.33.207
Abstract: Enzyme linked immunosorbent assay (ELISA) was applied to detect the antibody of Japanese flounder (Paralichthys olivaceus) against lymphocystis disease virus (LCDV). Purified Japanese flounder LCDV was used as a capture antigen of ELISA. Monitoring the immune response of Japanese flounder to LCDV was done using the sera obtained from apparently healthy, LCD-diseased and LCD-recovered fish. ELISA absorbances of these three groups were clearly different. Using the serum with high ELISA absorbance, the optimum ELISA condition was set up. Apparently healthy fish were injected with inactivated LCDV. One to three months after the injection, the antibody against LCDV was detected by ELISA established for LCDV and their ELISA antibody titer increased during that period.
精製リンホシスチス病(LCD)ウイルスを ELISA 抗原として, ヒラメ健康魚, 発病魚および感染耐過魚の血中抗体検出を試みたところ, 群れごとに ELISA 吸光値に差が認められ, 値は上記の順に高かった。 感染耐過魚の血清を用い, 抗原および各抗血清の最適濃度ならびに ELISA のベースラインを決定した。 健康魚に不活化ウイルスを接種し, 最適 ELISA 条件下で血中抗体価を測定したところ, 接種1~3か月後に抗体価の上昇が観察され, 本 ELISA は免疫後の血中抗体価を測定するのに有効であると考えられた。
Type: article
URI: http://hdl.handle.net/2115/53149
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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