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魚類由来培養細胞の魚類 ノダウイルス感受性

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Title: 魚類由来培養細胞の魚類 ノダウイルス感受性
Other Titles: Susceptibility of fish cell lines against fish nodavirus
Authors: 渡辺, 研一1 Browse this author
吉水, 守2 Browse this author →KAKEN DB
Authors(alt): Watanabe, Ken-ichi1
Yoshimizu, Mamoru2
Keywords: Fish nodavirus
Viral nervous necrosis
Fish cell line
Issue Date: 15-Dec-1999
Publisher: 日本魚病学会
Journal Title: 魚病研究
Journal Title(alt): Fish pathology
Volume: 34
Issue: 4
Start Page: 213
End Page: 214
Publisher DOI: 10.3147/jsfp.34.213
Abstract: Susceptibility of 32 fish cell lines against fish nodaviruses from moribund barfin flounder (Verasper moseri, BFNNV), greasy grouper (Epinephelus tauvina, GGNNV) and striped jack (Pseudocaranx dentex, SJNNV) was investigated. Cytopathic effects were observed on SSN-1 cells inoculated with the 3 viruses at 15 or 20°C, and on SBK-2 and SK, both derived from sea bass (Lates calcarifer), inoculated with GGNNV. Viral infectivity of homogenized brain and eye of moribund barfin flounder and Japanese flounder (Paralichthys olivaceus) juveniles were 10 7.55~8.55 and 10 6.05~6.55, respectively and whole bodies of striped jack larvae were 10 8.80~>9.80 TCID50/g.
各種海産魚, 温水性淡水魚およびサケ科魚類由来培養細胞32種類のマツカワ, ヒトミハタおよびシマアジ病魚由来ノダウイルスに対する感受性を観察した。供試ノダウイルス全てに感受性を示した細胞は SSN-1 のみであったが, ヒトミハタ由来ウイルスは SBK-2, SK にも CPE を発現した。SSN-1 細胞を用いて測定したマツカワおよびヒラメ病稚魚の脳および眼, シマアジ病仔魚の全魚体のウイルス感染価は, マツカワで10 7.55~8.55, ヒラメで10 6.05~6.55, シマアジでは10 8.80~>9.80TCID50/gであった。
Type: article
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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