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Cooperative Synthesis of Ultra Long-Chain Fatty Acid and Ceramide during Keratinocyte Differentiation

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Title: Cooperative Synthesis of Ultra Long-Chain Fatty Acid and Ceramide during Keratinocyte Differentiation
Authors: Mizutani, Yukiko Browse this author →KAKEN DB
Sun, Hui Browse this author
Ohno, Yusuke Browse this author
Sassa, Takayuki Browse this author →KAKEN DB
Wakashima, Takeshi Browse this author
Obara, Mari Browse this author
Yuyama, Kohei Browse this author →KAKEN DB
Kihara, Akio Browse this author →KAKEN DB
Igarashi, Yasuyuki Browse this author →KAKEN DB
Issue Date: 27-Jun-2013
Publisher: Public Library of Science
Journal Title: Plos One
Volume: 8
Issue: 6
Publisher DOI: 10.1371/journal.pone.0067317
Abstract: The lipid lamellae in the stratum corneum is important for the epidermal permeability barrier. The lipid lamellae component ceramide (CER), comprising an ultra long-chain (ULC) fatty acid (FA) of >= 26 carbons (ULC CER), plays an essential role in barrier formation. ULC acyl-CoAs, produced by the FA elongase ELOVL4, are converted to ULC CERs by the CER synthase CERS3. In the presented study, we observed that ELOVL4 and CERS3 mRNAs increased during keratinocyte differentiation in vivo and in vitro. We also determined that peroxisome proliferator-activated receptor beta/delta is involved in the up-regulation of the mRNAs. Knockdown of CERS3 caused a reduction in the elongase activities toward ULC acyl-CoAs, suggesting that CERS3 positively regulates ULCFA. Thus, we reveal that the two key players in ULC CER production in epidermis, CERS3 and ELOVL4, are coordinately regulated at both the transcriptional and enzymatic levels.
Rights: http://creativecommons.org/licenses/by/3.0/
Type: article
URI: http://hdl.handle.net/2115/53154
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 五十嵐 靖之

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