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Cooperative Synthesis of Ultra Long-Chain Fatty Acid and Ceramide during Keratinocyte Differentiation
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| Title: | Cooperative Synthesis of Ultra Long-Chain Fatty Acid and Ceramide during Keratinocyte Differentiation |
| Authors: | Mizutani, Yukiko Browse this author →KAKEN DB | | Sun, Hui Browse this author | | Ohno, Yusuke Browse this author | | Sassa, Takayuki Browse this author →KAKEN DB | | Wakashima, Takeshi Browse this author | | Obara, Mari Browse this author | | Yuyama, Kohei Browse this author →KAKEN DB | | Kihara, Akio Browse this author →KAKEN DB | | Igarashi, Yasuyuki Browse this author →KAKEN DB |
| Issue Date: | 27-Jun-2013 |
| Publisher: | Public Library of Science |
| Journal Title: | Plos One |
| Volume: | 8 |
| Issue: | 6 |
| Start Page: | e67317 |
| Publisher DOI: | 10.1371/journal.pone.0067317 |
| Abstract: | The lipid lamellae in the stratum corneum is important for the epidermal permeability barrier. The lipid lamellae component ceramide (CER), comprising an ultra long-chain (ULC) fatty acid (FA) of >= 26 carbons (ULC CER), plays an essential role in barrier formation. ULC acyl-CoAs, produced by the FA elongase ELOVL4, are converted to ULC CERs by the CER synthase CERS3. In the presented study, we observed that ELOVL4 and CERS3 mRNAs increased during keratinocyte differentiation in vivo and in vitro. We also determined that peroxisome proliferator-activated receptor beta/delta is involved in the up-regulation of the mRNAs. Knockdown of CERS3 caused a reduction in the elongase activities toward ULC acyl-CoAs, suggesting that CERS3 positively regulates ULCFA. Thus, we reveal that the two key players in ULC CER production in epidermis, CERS3 and ELOVL4, are coordinately regulated at both the transcriptional and enzymatic levels. |
| Rights: | http://creativecommons.org/licenses/by/3.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/53154 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 五十嵐 靖之
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