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Virulence change of infectious hematopoietic necrosis virus against rainbow trout Oncorhynchus mykiss with viral molecular evolution

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Title: Virulence change of infectious hematopoietic necrosis virus against rainbow trout Oncorhynchus mykiss with viral molecular evolution
Other Titles: 分子進化に伴い変化するIHNVの病原性
Authors: Mochizuki, Mamiko1 Browse this author
Kim, Hyoung Jun2 Browse this author
Kasai, Hisae3 Browse this author →KAKEN DB
Nishizawa, Toyohiko4 Browse this author →KAKEN DB
Yoshimizu, Mamoru5 Browse this author →KAKEN DB
Authors(alt): 望月, 万美子1
金, 亨埈2
笠井, 久会3
西澤, 豊彦4
吉水, 守5
Keywords: Infectious hematopoietic necrosis virus
IHNV
Virulence
Rainbow trout
Phylogeny
Nucleotide diversity
Issue Date: 15-Dec-2009
Publisher: 日本魚病学会
Journal Title: 魚病研究
Journal Title(alt): Fish pathology
Volume: 44
Issue: 4
Start Page: 159
End Page: 165
Publisher DOI: 10.3147/jsfp.44.159
Abstract: Infectious hematopoietic necrosis virus (IHNV) is the causative agent of IHN, one of the most serious viral diseases of salmonid fish. A total of five major genogroups including JRt for Asian isolates were confirmed among worldwide isolates based on glycoprotein (G) gene nucleotide (nt) sequences. The present study revealed existence of new two lineages, JRt Shizuoka and JRt Nagano, in the genogroup JRt by addition of new isolates obtained in 2006. The maximum nt diversity of G gene within JRt Shizuoka or JRt Nagano lineage was 6.3% or 3.5%, while that between JRt Shizuoka and JRt Nagano lineages was 7.0%. To evaluate influence of the evolutional divergence to virulence of IHNV, experimental challenges to rainbow trout Oncorhynchus mykiss were conducted by bath exposure at 10^4 TCID50/mL of RtShiz06s and RtShiz06a (JRt Shizuoka lineage), RtNag96 and RtNag06a (JRt Nagano lineage), and ChAb76 (a representative of the genogroup U). Distinct difference was observed in IHNV virulence to rainbow trout, i.e. the highest virulence was in RtShiz06s and RtShiz06a (≥ 76% of mortalities), and subsequently in RtNag96 and RtNag06a (20-40%), but scarcely any virulence in ChAb76 (≤ 10%). Thus it was suggested that nt diversity of Japanese IHNV continued rapidly with changing its virulence in rainbow trout farm environments.
2006年に分離されたIHNV日本株のGタンパク質遺伝子を解析した結果、IHNV日本株には静岡系および長野系が存在し、これらは日本のニジマス養殖環境下で今なお進化していることが示された。2006年分離株のニジマスに対する病原性を比較した結果、静岡系株での累積死亡率は76%以上であったが、長野系株では20-40%、また比較に用いた1976年の分離株では10%以下と、各株の病原性に差異が認められた。従って、ニジマス養殖環境下でのIHNVの分子進化に伴いIHNVのニジマスに対する病原性も変化していると考えられた。
Type: article
URI: http://hdl.handle.net/2115/53174
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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