HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >

Fish immunization using a synthetic double-stranded RNA Poly(I:C), an interferon inducer, offers protection against RGNNV, a fish nodavirus

Files in This Item:
article152.pdf282.32 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/53184

Title: Fish immunization using a synthetic double-stranded RNA Poly(I:C), an interferon inducer, offers protection against RGNNV, a fish nodavirus
Authors: Nishizawa, Toyohiko Browse this author →KAKEN DB
Takami, Ikuo Browse this author
Kokawa, Yuki Browse this author
Yoshimizu, Mamoru Browse this author →KAKEN DB
Keywords: Poly(I:C)
Immunization
Fish nodavirus
Viral nervous necrosis
VNN
Sevenband grouper
Vaccine
Antiviral state
Issue Date: 12-Feb-2009
Publisher: Inter-Research
Journal Title: Diseases of aquatic organisms
Volume: 83
Issue: 2
Start Page: 115
End Page: 122
Publisher DOI: 10.3354/dao02001
PMID: 19326792
Abstract: Viral nervous necrosis (VNN), caused by a fish nodavirus, is one of the most serious fish diseases worldwide. Here we report a unique vaccination method in sevenband grouper Epinephelus septemfasciatus using a synthetic double-stranded RNA polyinosinic polycytidylic acid (Poly(I:C)), an interferon inducer, followed by challenge with a live fish nodavirus. Fish injected with Poly(I:C) at 200 µg fish(-1) were highly protected from artificial challenge with red-spotted grouper nervous necrosis virus (RGNNV) (relative percentage survival, RPS: 100%), and specific antibodies against RGNNV were detected in sera from survivors. Moreover, the surviving fish were protected from rechallenge with RGNNV (relative percent survival RPS: 100%). Thus, it was confirmed that specific immunity against RGNNV was established in sevenband grouper by injection with live RGNNV following Poly(I:C) administration. Antiviral state was induced in fish by injection with Poly(I:C) at ≥50 µg fish(-1), but no toxic response was observed in the fish even if Poly(I:C) was injected at a dose of 200 µg fish(-1). In fish injected with Poly(I:C) at 200 µg fish(-1), a high level of antiviral state of > 90% RPS against RGNNV challenge lasted for at least 4 d after Poly(I:C) injection. However, no curative effect by Poly(I:C) injection was observed in fish already infected with RGNNV. It is considered that the present immunization method using Poly(I:C) followed by a live virus injection could offer protection against various viral infections in a broader range of fish species.
Rights: © 2009 Inter-Research
Type: article
URI: http://hdl.handle.net/2115/53184
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University