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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Fish immunization using a synthetic double-stranded RNA Poly(I:C), an interferon inducer, offers protection against RGNNV, a fish nodavirus
| Title: | Fish immunization using a synthetic double-stranded RNA Poly(I:C), an interferon inducer, offers protection against RGNNV, a fish nodavirus |
| Authors: | Nishizawa, Toyohiko Browse this author →KAKEN DB | | Takami, Ikuo Browse this author | | Kokawa, Yuki Browse this author | | Yoshimizu, Mamoru Browse this author →KAKEN DB |
| Keywords: | Poly(I:C) | | Immunization | | Fish nodavirus | | Viral nervous necrosis | | VNN | | Sevenband grouper | | Vaccine | | Antiviral state |
| Issue Date: | 12-Feb-2009 |
| Publisher: | Inter-Research |
| Journal Title: | Diseases of aquatic organisms |
| Volume: | 83 |
| Issue: | 2 |
| Start Page: | 115 |
| End Page: | 122 |
| Publisher DOI: | 10.3354/dao02001 |
| PMID: | 19326792 |
| Abstract: | Viral nervous necrosis (VNN), caused by a fish nodavirus, is one of the most serious fish diseases worldwide. Here we report a unique vaccination method in sevenband grouper Epinephelus septemfasciatus using a synthetic double-stranded RNA polyinosinic polycytidylic acid (Poly(I:C)), an interferon inducer, followed by challenge with a live fish nodavirus. Fish injected with Poly(I:C) at 200 µg fish(-1) were highly protected from artificial challenge with red-spotted grouper nervous necrosis virus (RGNNV) (relative percentage survival, RPS: 100%), and specific antibodies against RGNNV were detected in sera from survivors. Moreover, the surviving fish were protected from rechallenge with RGNNV (relative percent survival RPS: 100%). Thus, it was confirmed that specific immunity against RGNNV was established in sevenband grouper by injection with live RGNNV following Poly(I:C) administration. Antiviral state was induced in fish by injection with Poly(I:C) at ≥50 µg fish(-1), but no toxic response was observed in the fish even if Poly(I:C) was injected at a dose of 200 µg fish(-1). In fish injected with Poly(I:C) at 200 µg fish(-1), a high level of antiviral state of > 90% RPS against RGNNV challenge lasted for at least 4 d after Poly(I:C) injection. However, no curative effect by Poly(I:C) injection was observed in fish already infected with RGNNV. It is considered that the present immunization method using Poly(I:C) followed by a live virus injection could offer protection against various viral infections in a broader range of fish species. |
| Rights: | © 2009 Inter-Research |
| Type: | article |
| URI: | http://hdl.handle.net/2115/53184 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 吉水 守
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