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Hokkaido University Collection of Scholarly and Academic Papers >
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Existence of non-agglutinating Aeromonas salmonicida subsp. salmonicida in strains isolated from salmonids in Yamagata Prefecture, Japan
| Title: | Existence of non-agglutinating Aeromonas salmonicida subsp. salmonicida in strains isolated from salmonids in Yamagata Prefecture, Japan |
| Other Titles: | 山形県において分離された自発凝集性を示さないAeromonas salmonicida |
| Authors: | Nomura, Tetsuichi1 Browse this author | | Yoshimizu, Mamoru2 Browse this author →KAKEN DB | | Moki, Shouzo3 Browse this author | | Ezura, Yoshio4 Browse this author →KAKEN DB |
| Authors(alt): | 野村, 哲一1 | | 吉水, 守2 | | 茂木, 省三3 | | 絵面, 良男4 |
| Keywords: | Aeromonas salmonicida | | せっそう病 | | 自発凝集 | | 山形 | | 北海道 |
| Issue Date: | Mar-1994 |
| Publisher: | 北海道さけ・ますふ化場 |
| Journal Title: | 北海道さけ・ますふ化場研究報告 |
| Journal Title(alt): | Scientific reports of the Hokkaido Salmon Hatchery |
| Volume: | 48 |
| Start Page: | 23 |
| End Page: | 29 |
| Abstract: | Auto-agglutination, protein staining of colonies and protein pattern of A-layer in thirty three isolates of Aeromonas salmonicida from salmonids in various parts of Yamagata Prefecture and Hokkaido were studied. The strains isolated in Yamagata Prefecture did not showed auto-agglutination in broth, colony colour was mainly grey-white on TSA-C and NA-C medium, and there was no 50 kDa. major protein band in the A-layer with SDS-PAGE. It was revealed from the results that non-agglutinating A. salmonicida exist in the strains isolated in Yamagata Prefecture. While there were no strains isolated in Hokkaido which were non-agglutinating. We suspect that the reason for the existence of non-agglutinating strains in Yamagata Prefecture may depend on the temperature at which this bacterium was isolated. | | 山形県で分離された23株および北海道で分離された10株のAeromonas salmonicidaについて，液体培地での自発凝集性，Coomassie brilliant blueを添加した寒天培地上に発育したコロニーへの色素の吸着およびSDS-PAGEによるA-layerのタンパク組成を検討した．山形県で分離した株中4株は液体培地中で弱い自発凝集性を示し，寒天培地に出現したコロニーは主として色素の吸着を示さないコロニーであったが，一部のコロニーは吸着を示した．他の19株は全て自発凝集性を示さず，出現コロニーに色素の吸着は認められなかった．SDS-PAGEでは山形県での分離株は，北海道での分離株に見られた50kDa.の主要なタンパク質の存在は認められないか，微量であった．以上の結果から山形県での分離株には自発凝集を示さないA. salmonicidaが存在することが明らかになった． |
| Type: | article |
| URI: | http://hdl.handle.net/2115/53244 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 吉水 守
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