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Isolation and characterization of human breast cancer cells with SOX2 promoter activity
| Title: | Isolation and characterization of human breast cancer cells with SOX2 promoter activity |
| Authors: | Liang, Shanshan Browse this author | | Furuhashi, Masako Browse this author | | Nakane, Rie Browse this author | | Nakazawa, Seitaro Browse this author | | Goudarzi, Houman Browse this author →KAKEN DB | | Hamada, Jun-ichi Browse this author →KAKEN DB | | Iizasa, Hisashi Browse this author →KAKEN DB |
| Keywords: | SOX2 promoter | | Transcription factor reporter | | Cancer stem cells | | Stemness |
| Issue Date: | 26-Jul-2013 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and biophysical research communications |
| Volume: | 437 |
| Issue: | 2 |
| Start Page: | 205 |
| End Page: | 211 |
| Publisher DOI: | 10.1016/j.bbrc.2013.06.038 |
| PMID: | 23796710 |
| Abstract: | Sex determining region Y-box 2 (SOX2) is well known as one of the "sternness" factors and is often expressed in cancers including breast cancer. In this study, we developed a reporter system using fluorescent protein driven by the promoter for SOX2 gene to detect and isolate living SOX2-positive cells. Using this system, we determined that SOX2 promoter activities were well correlated with SOX2 mRNA expression levels in 5 breast cancer cell lines, and that the cell population with positive SOX2 promoter activity (pSp-T+) isolated from one of the 5 cell lines, MCF-7 cells, showed a high SOX2 protein expression and high sphere-forming activity compared with very low promoter activity (pSp-Tlow/-). The pSp-T(+)pbpulation expressed higher mRNA levels of several stemness-related genes such as CD44, ABCB1, NANOG and TWIST] than the pSp-Tlow/- population whereas the two populations expressed CD24 at similar levels. These results suggest that the cell population with SOX2 promoter activity contains cancer stem cell (CSC)-like cells which show different expression profiles from those of CSC-marker genes previously recognized in human breast cancers. (C) 2013 Elsevier Inc. All rights reserved. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/53246 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 飯笹 久
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