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Isolation and characterization of human breast cancer cells with SOX2 promoter activity

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Title: Isolation and characterization of human breast cancer cells with SOX2 promoter activity
Authors: Liang, Shanshan Browse this author
Furuhashi, Masako Browse this author
Nakane, Rie Browse this author
Nakazawa, Seitaro Browse this author
Goudarzi, Houman Browse this author →KAKEN DB
Hamada, Jun-ichi Browse this author →KAKEN DB
Iizasa, Hisashi Browse this author →KAKEN DB
Keywords: SOX2 promoter
Transcription factor reporter
Cancer stem cells
Issue Date: 26-Jul-2013
Publisher: Elsevier
Journal Title: Biochemical and biophysical research communications
Volume: 437
Issue: 2
Start Page: 205
End Page: 211
Publisher DOI: 10.1016/j.bbrc.2013.06.038
PMID: 23796710
Abstract: Sex determining region Y-box 2 (SOX2) is well known as one of the "sternness" factors and is often expressed in cancers including breast cancer. In this study, we developed a reporter system using fluorescent protein driven by the promoter for SOX2 gene to detect and isolate living SOX2-positive cells. Using this system, we determined that SOX2 promoter activities were well correlated with SOX2 mRNA expression levels in 5 breast cancer cell lines, and that the cell population with positive SOX2 promoter activity (pSp-T+) isolated from one of the 5 cell lines, MCF-7 cells, showed a high SOX2 protein expression and high sphere-forming activity compared with very low promoter activity (pSp-Tlow/-). The pSp-T(+)pbpulation expressed higher mRNA levels of several stemness-related genes such as CD44, ABCB1, NANOG and TWIST] than the pSp-Tlow/- population whereas the two populations expressed CD24 at similar levels. These results suggest that the cell population with SOX2 promoter activity contains cancer stem cell (CSC)-like cells which show different expression profiles from those of CSC-marker genes previously recognized in human breast cancers. (C) 2013 Elsevier Inc. All rights reserved.
Type: article (author version)
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 飯笹 久

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