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Hokkaido University Collection of Scholarly and Academic Papers >
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Regulation of neuropeptide Y Y1 receptor expression by bone morphogenetic protein 2 in C2C12 myoblasts
| Title: | Regulation of neuropeptide Y Y1 receptor expression by bone morphogenetic protein 2 in C2C12 myoblasts |
| Authors: | Kurebayashi, Naoko Browse this author | | Sato, Mari Browse this author →KAKEN DB | | Fujisawa, Toshiaki Browse this author →KAKEN DB | | Fukushima, Kazuaki Browse this author →KAKEN DB | | Tamura, Masato Browse this author →KAKEN DB |
| Keywords: | Osteoblasts | | Bone morphogenetic protein 2 | | Neuropeptide Y | | Y1 receptor |
| Issue Date: | 4-Oct-2013 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 439 |
| Issue: | 4 |
| Start Page: | 506 |
| End Page: | 510 |
| Publisher DOI: | 10.1016/j.bbrc.2013.09.014. |
| PMID: | 24025680 |
| Abstract: | The neuropeptide Y (NPY) system is known as one of the major neural signaling pathways. NPY, produced by peripheral tissues including osteoblasts, is known to bind to the Y1 receptor. Recently, osteoblast-specific Y1 receptor knockout mice were developed and were found to have a high bone mass phenotype, indicating a role for the NPY-Y1 receptor axis as a regulator of bone homeostasis. However, regulation of Y1 receptor expression during osteoblastic differentiation remains unexplored. In the present study, we examined the role of bone morphogenetic protein (BMP) 2 signaling in regulating Y1 receptor expression. In C2C12 cells, expression of Y1 receptor mRNA was induced by BMP2. This induction was also observed after co-transfection with Smad1 and Smad4, the intracellular signaling molecules of the BMP2 signaling pathway. In a transfection assay, Smad1/4 up-regulated transcriptional activity through interaction with the Y1 receptor gene promoter. Following transfection of MC3T3-E1 cells with siRNA for the Y1 receptor, the expression of alkaline phosphatase, osteocalcin, Runx2 and osterix were increased. These results show that BMP2 signaling regulates Y1 receptor gene expression, and raises the possibility that NPY acts in osteoblasts via an autocrine mechanism. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/53258 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 田村 正人
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