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Regulation of neuropeptide Y Y1 receptor expression by bone morphogenetic protein 2 in C2C12 myoblasts

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Title: Regulation of neuropeptide Y Y1 receptor expression by bone morphogenetic protein 2 in C2C12 myoblasts
Authors: Kurebayashi, Naoko Browse this author
Sato, Mari Browse this author →KAKEN DB
Fujisawa, Toshiaki Browse this author →KAKEN DB
Fukushima, Kazuaki Browse this author →KAKEN DB
Tamura, Masato Browse this author →KAKEN DB
Keywords: Osteoblasts
Bone morphogenetic protein 2
Neuropeptide Y
Y1 receptor
Issue Date: 4-Oct-2013
Publisher: Elsevier
Journal Title: Biochemical and Biophysical Research Communications
Volume: 439
Issue: 4
Start Page: 506
End Page: 510
Publisher DOI: 10.1016/j.bbrc.2013.09.014.
PMID: 24025680
Abstract: The neuropeptide Y (NPY) system is known as one of the major neural signaling pathways. NPY, produced by peripheral tissues including osteoblasts, is known to bind to the Y1 receptor. Recently, osteoblast-specific Y1 receptor knockout mice were developed and were found to have a high bone mass phenotype, indicating a role for the NPY-Y1 receptor axis as a regulator of bone homeostasis. However, regulation of Y1 receptor expression during osteoblastic differentiation remains unexplored. In the present study, we examined the role of bone morphogenetic protein (BMP) 2 signaling in regulating Y1 receptor expression. In C2C12 cells, expression of Y1 receptor mRNA was induced by BMP2. This induction was also observed after co-transfection with Smad1 and Smad4, the intracellular signaling molecules of the BMP2 signaling pathway. In a transfection assay, Smad1/4 up-regulated transcriptional activity through interaction with the Y1 receptor gene promoter. Following transfection of MC3T3-E1 cells with siRNA for the Y1 receptor, the expression of alkaline phosphatase, osteocalcin, Runx2 and osterix were increased. These results show that BMP2 signaling regulates Y1 receptor gene expression, and raises the possibility that NPY acts in osteoblasts via an autocrine mechanism.
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田村 正人

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