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Hokkaido University Collection of Scholarly and Academic Papers >
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Ca2+-binding properties and regulatory roles of lobster troponin C sites II and IV
| Title: | Ca2+-binding properties and regulatory roles of lobster troponin C sites II and IV |
| Authors: | Tanaka, Hiroyuki Browse this author →KAKEN DB | | Takahashi, Hiroki Browse this author →KAKEN DB | | Ojima, Takao Browse this author →KAKEN DB |
| Keywords: | Troponin | | Muscle | | Ca2+-regulation | | Invertebrate | | Arthropod | | Isoform |
| Issue Date: | 19-Aug-2013 |
| Publisher: | Elsevier science bv |
| Journal Title: | Febs letters |
| Volume: | 587 |
| Issue: | 16 |
| Start Page: | 2612 |
| End Page: | 2616 |
| Publisher DOI: | 10.1016/j.febslet.2013.06.042 |
| PMID: | 23831063 |
| Abstract: | Invertebrate troponin C typically contains Ca2+-specific binding sites, sites II and IV, in the N- and C-terminal domains, respectively. To investigate the roles of these sites for Ca2+-dependent regulation of muscle contraction, we generated lobster troponin C mutants, and analyzed their Ca2+-binding properties and regulatory effects on actomyosin-tropomyosin Mg-ATPase activity. The results suggest that Ca2+ binding to site IV is responsible for regulation at relatively low Ca2+ concentrations, while site II has an essential role in full activation at higher Ca2+ concentrations. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/53273 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 田中 啓之
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