Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Evaluation of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a screening method for the detection of influenza viruses in the fecal materials of water birds.
Title: | Evaluation of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a screening method for the detection of influenza viruses in the fecal materials of water birds. |
Authors: | Yoshida, Hiromi Browse this author | Sakoda, Yoshihiro Browse this author →KAKEN DB | Endo, Mayumi Browse this author | Motoshima, Masayuki Browse this author | Yoshino, Fumi Browse this author | Yamamoto, Naoki Browse this author | Okamatsu, Masatoshi Browse this author →KAKEN DB | Soejima, Takahiro Browse this author | Senba, Syouhei Browse this author | Kanda, Hidetoshi Browse this author | Kida, Hiroshi Browse this author →KAKEN DB |
Issue Date: | Jun-2011 |
Publisher: | the Japanese Society of Veterinary Science |
Journal Title: | The Journal of veterinary medical science |
Volume: | 73 |
Issue: | 6 |
Start Page: | 753 |
End Page: | 758 |
Publisher DOI: | 10.1292/jvms.10-0505 |
PMID: | 21289470 |
Abstract: | Migratory water birds are a natural reservoir for influenza A viruses. Viruses replicate in the intestines of ducks and are shed with the fecal materials. Virus isolation from collected fecal materials, therefore, is an integral part of the surveillance of avian influenza in water birds. In the present study, reverse transcription loop-mediated isothermal amplification (RT-LAMP) was assessed for its usefulness in detecting the RNA of influenza A viruses in fecal materials. It was found that, RT-LAMP specifically and sensitively detects the matrix gene of influenza A viruses. Influenza A viruses were isolated from the fecal materials in which viral RNA were detected by RT-LAMP in 35 min. The present findings indicate that RT-LAMP is useful as a high throughput screening method for field samples prior to virus isolation, allowing the processing of hundreds of samples per day. |
Type: | article |
URI: | http://hdl.handle.net/2115/53281 |
Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 喜田 宏
|