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Evaluation of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a screening method for the detection of influenza viruses in the fecal materials of water birds.

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Title: Evaluation of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a screening method for the detection of influenza viruses in the fecal materials of water birds.
Authors: Yoshida, Hiromi Browse this author
Sakoda, Yoshihiro Browse this author →KAKEN DB
Endo, Mayumi Browse this author
Motoshima, Masayuki Browse this author
Yoshino, Fumi Browse this author
Yamamoto, Naoki Browse this author
Okamatsu, Masatoshi Browse this author →KAKEN DB
Soejima, Takahiro Browse this author
Senba, Syouhei Browse this author
Kanda, Hidetoshi Browse this author
Kida, Hiroshi Browse this author →KAKEN DB
Issue Date: Jun-2011
Publisher: the Japanese Society of Veterinary Science
Journal Title: The Journal of veterinary medical science
Volume: 73
Issue: 6
Start Page: 753
End Page: 758
Publisher DOI: 10.1292/jvms.10-0505
PMID: 21289470
Abstract: Migratory water birds are a natural reservoir for influenza A viruses. Viruses replicate in the intestines of ducks and are shed with the fecal materials. Virus isolation from collected fecal materials, therefore, is an integral part of the surveillance of avian influenza in water birds. In the present study, reverse transcription loop-mediated isothermal amplification (RT-LAMP) was assessed for its usefulness in detecting the RNA of influenza A viruses in fecal materials. It was found that, RT-LAMP specifically and sensitively detects the matrix gene of influenza A viruses. Influenza A viruses were isolated from the fecal materials in which viral RNA were detected by RT-LAMP in 35 min. The present findings indicate that RT-LAMP is useful as a high throughput screening method for field samples prior to virus isolation, allowing the processing of hundreds of samples per day.
Type: article
URI: http://hdl.handle.net/2115/53281
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 喜田 宏

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