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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
RT-PCRと細胞培養法を組合せた伝染性造血器壊死症ウイルス(IHNV)の検出
| Title: | RT-PCRと細胞培養法を組合せた伝染性造血器壊死症ウイルス(IHNV)の検出 |
| Other Titles: | Detection of infectious hematopoietic necrosis virus using RT-PCR combined with tissue culture |
| Authors: | 吉仲, 桃子1 Browse this author | | 堀, 友花2 Browse this author | | 本西, 晃3 Browse this author | | 河西, 一彦4 Browse this author | | 山本, 淳5 Browse this author | | 鈴木, 邦雄6 Browse this author | | 宇賀神, 光男7 Browse this author | | 野村, 哲一8 Browse this author | | 吉水, 守9 Browse this author →KAKEN DB |
| Authors(alt): | Yoshinaka, Toko1 | | Hori, Yuuka2 | | Motonishi, Akira3 | | Kasai, Kazuhiko4 | | Yamamoto, Atsushi5 | | Suzuki, Kunio6 | | Ugazin, Mitsuo7 | | Nomura, Tetsuichi8 | | Yoshimizu, Mamoru9 |
| Keywords: | Virus detection | | IHN | | IHNV | | RT-PCR |
| Issue Date: | Dec-1998 |
| Publisher: | 水産庁さけ・ます資源管理センター |
| Journal Title: | さけ・ます資源管理センタ-研究報告 |
| Journal Title(alt): | Bulletin of the National Salmon Resources Center |
| Volume: | 1 |
| Start Page: | 29 |
| End Page: | 34 |
| Abstract: | Direct RT-PCR, tissue culture and RT-PCR combined with tissue culture were compared for the IHNV inspection in juvenile masu (Oncorhynchus masou) and sockeye salmon (O. nerka). The detection rate of IHNV from artificially infected rainbow trout (O. mykiss) was also compared by using tissue culture and RT-PCR combined with tissue culture. IHNV-infected salmonid fish were detected highly using RT-PCR combined with tissue culture. This method restrained inhibition of IHNV RNA extraction, because IHNV was replicated in the inoculated cells and inhibition of IHNV RNA extraction was diluted. The RT-PCR combined with tissue culture is a good method for the routine examination of IHNV. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/53386 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 吉水 守
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