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Diethyl phthalate enhances expression of SIRT1 and DNMT3a during apoptosis in PC12 cells

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Title: Diethyl phthalate enhances expression of SIRT1 and DNMT3a during apoptosis in PC12 cells
Authors: Sun, Yongkun Browse this author
Guo, Zhikun Browse this author
Iku, Shouhei Browse this author
Saito, Takeshi Browse this author →KAKEN DB
Kurasaki, Masaaki Browse this author →KAKEN DB
Keywords: apoptosis
DNA methylation
endocrine disrupter
Issue Date: Dec-2013
Publisher: John Wiley & Sons
Journal Title: Journal of Applied Toxicology
Volume: 33
Issue: 12
Start Page: 1484
End Page: 1492
Publisher DOI: 10.1002/jat.2816
PMID: 22968747
Abstract: Diethyl phthalate (DEP) works as a phthalate plasticizer and is ubiquitously used in personal care products, cosmetics, medical equipment and pharmaceutical coating. DEP is considered a potential endocrine disruptor. Previously we found DEP-enhanced apoptosis induced by serum deprivation in PC12 cells. However, the relationship between DEP and longevity-related factors, sirtuins and epigenetic factors (e.g. DNA methyltransferases) remains unclear, because genome modification caused by chemical toxicity, sirtuins and epigenetic factors have played key roles in abnormal metabolism and development. Here, we investigate whether DEP affects sirtuins (SIRT1 and SIRT2) and methyltranferases (DNMT1 and DNMT3a) on the apoptosis of PC12 cells. We found that DNMT3a was significantly decreased by serum deprivation. However, DNMT3a, DNMT3b and SIRT1 were significantly increased under the enhancement of apoptosis induced by serum deprivation. These results suggest that SIRT1, DNMT3a and DNMT3b play multiple and complex roles in different apoptotic stages. Our results showed DEP triggered epigenetic factors on PC12 cells apoptosis under nutrition stress. Finally, our results suggest that monitoring epigenetic factors such as DNMT3a, DNMT3b and SIRT1 could be a useful tool for chemical toxicity risk assessment.
Rights: Copyright © 2012 John Wiley & Sons, Ltd. Published online in Wiley Online Library: 11 September 2012
Type: article (author version)
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 藏崎 正明

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