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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Environmental Science / Faculty of Environmental Earth Science >
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Mechanism of the toxicity induced by natural humic acid on human vascular endothelial cells
| Title: | Mechanism of the toxicity induced by natural humic acid on human vascular endothelial cells |
| Authors: | Kihara, Yusuke Browse this author | | Yustiawati Browse this author | | Tanaka, Masato Browse this author | | Gumiri, Sulmin Browse this author | | Ardianor Browse this author | | Hosokawa, Toshiyuki Browse this author →KAKEN DB | | Tanaka, Shunitz Browse this author →KAKEN DB | | Saito, Takeshi Browse this author →KAKEN DB | | Kurasaki, Masaaki Browse this author →KAKEN DB |
| Keywords: | humic acid | | tropical peatland | | apoptosis | | endothelial cell | | oxidative stress |
| Issue Date: | Aug-2014 |
| Publisher: | Wiley |
| Journal Title: | Environmental Toxicology |
| Volume: | 29 |
| Issue: | 8 |
| Start Page: | 916 |
| End Page: | 925 |
| Publisher DOI: | 10.1002/tox.21819 |
| PMID: | 23042718 |
| Abstract: | Humic acid (HA), a group of high-molecular weight organic compounds characterized by an ability to bind heavy metals, is normally found in natural water. Although the impairment of vascular endothelial cells in the presence of humic substances has been reported to be involved in some diseases, the mechanisms responsible for this involvement remain unclear. In this study, we examined the cytotoxicity of HA obtained from peatland in Central Kalimantan, Indonesia, to human vascular endothelial cells, as well as the mechanisms behind these effects. It was found that 50 mg/L HA showed cytotoxicity, which we considered to be mediated by apoptosis through the mitochondrial pathway because of an increase in the expression of caspases 6 and 9 in response to HA administration. In addition, this cytotoxicity was enhanced when cells in this experimental system were exposed to oxidative stress, while it was decreased by the addition of vitamin C. Thus, we conclude that the apoptosis induced by HA depends upon oxidative stress. Furthermore, an iron chelator, DFO, showed a tendency to decrease HA-induced cytotoxicity, suggesting that iron may potentially mediate HA-induced oxidative stress. In conclusion, long-term consumption of HA-rich water obtained from our study area may cause damage to endothelial cells and subsequent chronic health problems. © 2012 Wiley Periodicals, Inc. Environ Toxicol, 2012. |
| Rights: | This is the pre-peer reviewed version of the following article: Kihara, Y., Yustiawati, , Tanaka, M., Gumiri, S., Ardianor, , Hosokawa, T., Tanaka, S., Saito, T. and Kurasaki, M. (2014), Mechanism of the toxicity induced by natural humic acid on human vascular endothelial cells. Environ. Toxicol, 29: 916-925., which has been published in final form at https://doi.org/10.1002/tox.21819. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/53585 |
| Appears in Collections: | 環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 藏崎 正明
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