| Title: | Altered distribution of bone matrix proteins and defective bone mineralization in klotho-deficient mice |
| Authors: | Sasaki, Muneteru Browse this author |
| Hasegawa, Tomoka Browse this author |
| Yamada, Tamaki Browse this author |
| Hongo, Hiromi Browse this author |
| Luiz de Freitas, Paulo Henrique Browse this author |
| Suzuki, Reiko Browse this author →KAKEN DB |
| Yamamoto, Tomomaya Browse this author |
| Tabata, Chihiro Browse this author |
| Toyosawa, Satoru Browse this author →KAKEN DB |
| Yamamoto, Tsuneyuki Browse this author →KAKEN DB |
| Oda, Kimimitsu Browse this author →KAKEN DB |
| Li, Minqi Browse this author →KAKEN DB |
| Inoue, Nobuo Browse this author →KAKEN DB |
| Amizuka, Norio Browse this author →KAKEN DB |
| Keywords: | Klotho deficient mice |
| Osteocyte |
| DMP-1 |
| Osteocalcin |
| MGP |
| Issue Date: | Nov-2013 |
| Publisher: | Elsevier |
| Journal Title: | Bone |
| Volume: | 57 |
| Issue: | 1 |
| Start Page: | 206 |
| End Page: | 219 |
| Publisher DOI: | 10.1016/j.bone.2013.08.008 |
| PMID: | 23954506 |
| Abstract: | In an attempt to identify the histological properties of the klotho-deficient (kl/kl) bone matrix, bone mineralization and the localization of Ca2+-binding bone matrix proteins - osteocalcin, dentin matrix protein-1 (DMP-1) and matrix Gla protein (MGP) - were examined in kl/kl tibiae. While a widespread osteocalcin staining could be verified in the wild-type bone matrix, localization of the same protein in the kl/kl tibiae seemed rather restricted to osteocytes with only a faint staining of the whole bone matrix. In wild-type mice, MGP immunoreactivity was present at the junction between the epiphyseal bone and cartilage, and at the insertion of the cruciate ligaments. In kl/kl mice, however, MGP was seen around the cartilaginous cores of the metaphyseal trabeculae and in the periphery of some cells of the bone surface. DMP-1 was identified in the osteocytic canalicular system of wild-type tibiae, but in the kl/kl tibiae this protein was mostly found in the osteocytic lacunae and in the periphery of some cells of the bone surface. Mineralization of the kl/kl bone seemed somewhat defective, with broad unmineralized areas within its matrix. In these areas, mineralized osteocytes along with their lacunae and osteocytic cytoplasmic processes were found to have intense osteocalcin and DMP-1 staining. Taken together, it might be that the excessive production of Ca2+-binding molecules such as osteocalcin and DMP-1 by osteocytes concentrates mineralization around such cells, disturbing the completeness of mineralization in the kl/kl bone matrix. (C) 2013 Elsevier Inc. All rights reserved. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/53690 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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