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Altered distribution of bone matrix proteins and defective bone mineralization in klotho-deficient mice

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Title: Altered distribution of bone matrix proteins and defective bone mineralization in klotho-deficient mice
Authors: Sasaki, Muneteru Browse this author
Hasegawa, Tomoka Browse this author
Yamada, Tamaki Browse this author
Hongo, Hiromi Browse this author
Luiz de Freitas, Paulo Henrique Browse this author
Suzuki, Reiko Browse this author →KAKEN DB
Yamamoto, Tomomaya Browse this author
Tabata, Chihiro Browse this author
Toyosawa, Satoru Browse this author →KAKEN DB
Yamamoto, Tsuneyuki Browse this author →KAKEN DB
Oda, Kimimitsu Browse this author →KAKEN DB
Li, Minqi Browse this author →KAKEN DB
Inoue, Nobuo Browse this author →KAKEN DB
Amizuka, Norio Browse this author →KAKEN DB
Keywords: Klotho deficient mice
Issue Date: Nov-2013
Publisher: Elsevier
Journal Title: Bone
Volume: 57
Issue: 1
Start Page: 206
End Page: 219
Publisher DOI: 10.1016/j.bone.2013.08.008
PMID: 23954506
Abstract: In an attempt to identify the histological properties of the klotho-deficient (kl/kl) bone matrix, bone mineralization and the localization of Ca2+-binding bone matrix proteins - osteocalcin, dentin matrix protein-1 (DMP-1) and matrix Gla protein (MGP) - were examined in kl/kl tibiae. While a widespread osteocalcin staining could be verified in the wild-type bone matrix, localization of the same protein in the kl/kl tibiae seemed rather restricted to osteocytes with only a faint staining of the whole bone matrix. In wild-type mice, MGP immunoreactivity was present at the junction between the epiphyseal bone and cartilage, and at the insertion of the cruciate ligaments. In kl/kl mice, however, MGP was seen around the cartilaginous cores of the metaphyseal trabeculae and in the periphery of some cells of the bone surface. DMP-1 was identified in the osteocytic canalicular system of wild-type tibiae, but in the kl/kl tibiae this protein was mostly found in the osteocytic lacunae and in the periphery of some cells of the bone surface. Mineralization of the kl/kl bone seemed somewhat defective, with broad unmineralized areas within its matrix. In these areas, mineralized osteocytes along with their lacunae and osteocytic cytoplasmic processes were found to have intense osteocalcin and DMP-1 staining. Taken together, it might be that the excessive production of Ca2+-binding molecules such as osteocalcin and DMP-1 by osteocytes concentrates mineralization around such cells, disturbing the completeness of mineralization in the kl/kl bone matrix. (C) 2013 Elsevier Inc. All rights reserved.
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 網塚 憲生

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