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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
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Hydrogels as feeder-free scaffolds for long-term self-renewal of mouse induced pluripotent stem (mips) cells.
| Title: | Hydrogels as feeder-free scaffolds for long-term self-renewal of mouse induced pluripotent stem (mips) cells. |
| Authors: | Yang, Jing Jing Browse this author | | Liu, Jian Fang Browse this author | | Kurokawa, Takayuki Browse this author →KAKEN DB | | Kitada, Kazuhiro Browse this author →KAKEN DB | | Gong, Jian Ping Browse this author →KAKEN DB |
| Issue Date: | Apr-2015 |
| Publisher: | John Wiley & Sons |
| Journal Title: | Journal of Tissue Engineering and Regenerative Medicine |
| Volume: | 9 |
| Issue: | 4 |
| Start Page: | 375 |
| End Page: | 388 |
| Publisher DOI: | 10.1002/term.1640 |
| PMID: | 23166055 |
| Abstract: | Expanding undifferentiated induced pluripotent stem (iPS) cells in vitro is a basic requirement for application of iPS cells in both fundamental research and clinical regeneration. In this study, we intended to establish a simple, low cost and efficient method for the long-term self-renewal of mouse induced pluripotent stem (miPS) cells without using feeder-cells and adhesive proteins. Three scaffolds were selected for the long-term subculture of miPS cells over two months starting from passages 14 to 29: 1) a gelatin coated polystyrene (Gelatin-PS) that is a widely used scaffold for self-renewal of mouse embryonic stem (mES) cells; 2) a neutral hydrogel poly(N,N-dimethylacrylamide) (PDMAAm); and 3) a negatively charged hydrogel poly(2-acrylamido-2-methyl-propane sulfonic acid sodium salt) (PNaAMPS). Each passaged miPS cells on these scaffolds were cryopreserved successfully and the revived cells showed high viability and proliferation. The passaged miPS cells maintained a high undifferentiated state on all three scaffolds and a high level of pluripotency by expressing differentiation markers in vitro and forming teratomas in SCID mice with derivatives of all three germ layers. Compared to Gelatin-PS, the two hydrogels exhibited much better self-renewal performance in terms of high proliferation rate and level of expression of undifferentiated gene markers as well as efficiency in pluripotent teratoma formation. Furthermore, the PNaAMPS hydrogel demonstrated a slightly higher efficiency and simpler operation of cell expansion than the PDMAAm hydrogel. To conclude, PNaAMPS hydrogel is an excellent feeder-free scaffold because of its simplicity, low cost and high efficiency in expanding a large number of miPS cells in vitro. Copyright © 2012 John Wiley & Sons, Ltd. |
| Rights: | This is the pre-peer-reviewed version of the following article: J Tissue Eng Regen Med. 2012 Nov 20, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/term.1640/abstract |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/53998 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 龔 剣萍 (Gong Jian Ping)
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