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Expression of O6-methylguanine DNA methyltransferase (MGMT) and immunohistochemical analysis of 12 pineal parenchymal tumors

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Title: Expression of O6-methylguanine DNA methyltransferase (MGMT) and immunohistochemical analysis of 12 pineal parenchymal tumors
Authors: Kanno, Hiromi Browse this author
Nishihara, Hiroshi Browse this author
Oikawa, Mitsuteru Browse this author
Ozaki, Yoshimaru Browse this author
Murata, Junichi Browse this author
Sawamura, Yutaka Browse this author
Kato, Masahito Browse this author
Kubota, Kanako Browse this author
Tanino, Mishie Browse this author
Kimura, Taichi Browse this author
Nagashima, Kazuo Browse this author
Itoh, Tamio Browse this author
Tanaka, Shinya Browse this author
Keywords: immunohistochemistry
MGMT
MIB-1
pinealoma
temozolomide
Issue Date: Dec-2012
Publisher: Blackwell Publishing
Journal Title: Neuropathology
Volume: 32
Issue: 6
Start Page: 647
End Page: 653
Publisher DOI: 10.1111/j.1440-1789.2012.01315.x
PMID: 22458700
Abstract: Pineal parenchymal tumors (PPTs) are rare neoplasms which occupy less than 1% of primary central nervous system tumors. Because of their rare incidence, the previous reports on PPTs are limited in number and the useful molecular markers for deciding the histological grading and even selecting chemotherapy are undetermined. In this study, we conducted immunohistochemical analysis of 12 PPT specimens, especially for expression of O6-methylguanine DNA methyltransferase (MGMT) to assess whether temozolomide (TMZ) could serve as a possible alternative therapy for PPTs. We analyzed 12 PPTs consisting of 3 pineocytomas, 6 pineal parenchymal tumors of intermediate differentiation (PPTIDs), and 3 pineoblastomas. Immunohistochemical analysis was performed using antibody against MGMT, synaptophysin, neurofilament protein (NF), p53, and NeuN. Immunohistochemically, 11 out of 12 cases were positive for MGMT. The mean MIB-1 labeling index was less than 1% in pineocytoma, 3.5% in PPTID, and 10.5% in pineoblastoma. All 12 cases were positive for synaptophysin and 11 cases except 1 PPTID case showed positive for NF. Nuclear staining of NeuN was negative in all cases although cytoplasmic stain of NeuN was observed in 5 cases. No case was positive for p53. Eleven out of 12 cases of PPTs demonstrated MGMT expression, suggesting chemoresistancy to TMZ treatment. This is the first report showing MGMT expression in PPTs. In addition, MIB-1 labeling index correlated with WHO grade, although the immunoreactivity of synaptophysin, NF, NeuN, and p53 did not correlate with the histological grade.
Rights: The definitive version is available at wileyonlinelibrary.com
Type: article (author version)
URI: http://hdl.handle.net/2115/54012
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 西原 広史

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