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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Environmental Science / Faculty of Environmental Earth Science >
Peer-reviewed Journal Articles, etc >
Aspartame-induced apoptosis in PC12 cells
| Title: | Aspartame-induced apoptosis in PC12 cells |
| Authors: | Horio, Yukari Browse this author | | Sun, Yongkun Browse this author | | Liu, Chuang Browse this author | | Saito, Takeshi Browse this author →KAKEN DB | | Kurasaki, Masaaki Browse this author →KAKEN DB |
| Keywords: | Aspartame | | Apoptosis | | Caspases | | AIF | | PC12 | | Cytochrome c |
| Issue Date: | Jan-2014 |
| Publisher: | Elsevier, B.V. |
| Journal Title: | Environmental Toxicology and Pharmacology |
| Volume: | 37 |
| Issue: | 1 |
| Start Page: | 158 |
| End Page: | 165 |
| Publisher DOI: | 10.1016/j.etap.2013.11.021 |
| PMID: | 24355796 |
| Abstract: | Aspartame is an artificial sweetner added to many low-calorie foods. The safety of aspartame remains controversial even though there are many studies on its risks. In this study, to understand the physiological effects of trace amounts of artificial sweetners on cells, the effects of aspartame on apoptosis were investigated using a PC12 cell system. In addition, the mechanism of apoptosis induced by aspartame in PC12 cells and effects on apoptotic factors such as cytochrome c, apoptosis-inducing factor, and caspase family proteins were studied by Western blotting and RT-PCR. Aspartame-induced apoptosis in PC12 cells in a dose-dependent manner. In addition, aspartame exposure increased the expressions of caspases 8 and 9, and cytochrome c. These results indicate that aspartame induces apoptosis mainly via mitochondrial pathway involved in apoptosis due to oxigen toxicity. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/54098 |
| Appears in Collections: | 環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 藏崎 正明
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