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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
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Renibacterium salmoninarum の表在抗原p57をELISA用抗原とした抗体検出
| Title: | Renibacterium salmoninarum の表在抗原p57をELISA用抗原とした抗体検出 |
| Other Titles: | Detection of specific antibodies against Renibacterium salmoninarum by ELISA with p57 protein as a capture antigen |
| Authors: | 松井, 崇憲1 Browse this author | | 大迫, 典久2 Browse this author →KAKEN DB | | 西澤, 豊彦3 Browse this author →KAKEN DB | | 吉水, 守4 Browse this author →KAKEN DB |
| Authors(alt): | Matsui, Takanori1 | | Oseko, Norihisa2 | | Nishizawa, Toyohiko3 | | Yoshimizu, Mamoru4 |
| Keywords: | antibody-detection ELISA | | Renibacterium salmoninarum | | BKD | | capture antigen | | O. mykiss | | O. masou |
| Issue Date: | 15-Jun-2010 |
| Publisher: | 日本魚病学会 |
| Journal Title: | 魚病研究 |
| Volume: | 45 |
| Issue: | 2 |
| Start Page: | 80 |
| End Page: | 83 |
| Publisher DOI: | 10.3147/jsfp.45.80 |
| Abstract: | We evaluated p57 of Renibacterium salmoninarum (R.s.) as a capture antigen for specific antibody-detection ELISA against R.s.. Using formalin-killed R.s. (FK-R.s.) as ELISA antigens, ELISA values (OD492 (nm)) of the negative control sera showed high values ranging from 0.12 to 1.59. These values were relatively lower than those of sera from the fish immunized with FK-R.s. (0.16-2.6), but seemed to be still high as negative control sera. Contrarily, when p57 was used as a capture antigen, ELISA values of the negative control sera were all 0.1 or less, while those of the sera from immunized fish ranged from 0.02 to 1.28. It was thus demonstrated that high background of ELISA was enabled to be reduced by p57 as ELISA antigens. | | R.s.表在抗原p57 のELISA用抗原への利用について検討した。ホルマリン死菌（FK-R.s.）固相化プレートでは，FK-R.s.およびPBS接種したサクラマスとニジマスの血清のELISA値は各々0.16～2.6と0.12～1.59で，FK-R.s.接種魚血清のELISA値はPBS接種魚血清に比べ高かったが，PBS接種魚血清の何れも0.1より高い値を示した。p57固相化プレートでは，FK-R.s.接種魚血清のELISA値は0.02～1.28であったが，PBS接種魚の血清は何れも0.1以下となった。p57をELISA抗原とすることで抗R.s.抗体の特異検出が可能になった。 |
| Type: | article |
| URI: | http://hdl.handle.net/2115/54118 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 吉水 守
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