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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
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Antibody detection against red sea bream iridovirus (RSIV) in yellowtail Seriola quinqueradiata using ELISA
| Title: | Antibody detection against red sea bream iridovirus (RSIV) in yellowtail Seriola quinqueradiata using ELISA |
| Other Titles: | ELISA法による抗RSIVブリ抗体の検出 |
| Authors: | Kwon, Se Ryun Browse this author | | Nishizawa, Toyohiko Browse this author →KAKEN DB | | Takami, Ikuo Browse this author | | Yoshimizu, Mamoru Browse this author →KAKEN DB |
| Keywords: | antibody detection | | ELISA | | red sea bream iridovirus | | yellowtail |
| Issue Date: | 15-Jun-2010 |
| Publisher: | The Japanese Society of Fish Pathology |
| Journal Title: | Fish Pathology |
| Volume: | 45 |
| Issue: | 2 |
| Start Page: | 73 |
| End Page: | 76 |
| Publisher DOI: | 10.3147/jsfp.45.73 |
| Abstract: | FBS components in viral culture fluid interfere with the detection of specific antibody in immunized fish. In the present study, we developed a method to eliminate the interference of FBS using a model of red sea bream iridovirus (RSIV) vaccine. In an experiment using yellowtail antiserum against BSA, which is a major component of FBS in cell culture media, antibodies against BSA were completely absorbed via incubation of antiserum in 50% FBS at 25 degrees C for 1 h. In antibody detection, ELISA with the sera of yellowtail surviving RSIV-challenge, antibodies against RSIV were detected, but those against FBS disappeared via the pre-treatment of the sera. Therefore, antibodies against RSIV are effectively detected using ELISA with the pre-treatment of fish sera. | | RSIV培養液を接種したブリからの抗RSIV抗体検出ELISA法について検討した。培養液に含まれるFBSの主要成分であるBSAに対する抗ブリ血清を用いた場合、血清に終濃度50％（v／v）となるようFBSを添加し25℃で1時間処理することで、血清中の抗BSA抗体が完全に吸収された。培養RSIV液で攻撃した生残ブリから得た血清を同様に処理することで、抗体検出ELISAにより抗RSIV抗体が検出できることが明らかになった。 |
| Type: | article |
| URI: | http://hdl.handle.net/2115/54120 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 吉水 守
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