HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >

Antibody detection against red sea bream iridovirus (RSIV) in yellowtail Seriola quinqueradiata using ELISA

Files in This Item:
article164.pdf623.61 kBPDFView/Open
Please use this identifier to cite or link to this item:

Title: Antibody detection against red sea bream iridovirus (RSIV) in yellowtail Seriola quinqueradiata using ELISA
Other Titles: ELISA法による抗RSIVブリ抗体の検出
Authors: Kwon, Se Ryun Browse this author
Nishizawa, Toyohiko Browse this author →KAKEN DB
Takami, Ikuo Browse this author
Yoshimizu, Mamoru Browse this author →KAKEN DB
Keywords: antibody detection
red sea bream iridovirus
Issue Date: 15-Jun-2010
Publisher: The Japanese Society of Fish Pathology
Journal Title: Fish Pathology
Volume: 45
Issue: 2
Start Page: 73
End Page: 76
Publisher DOI: 10.3147/jsfp.45.73
Abstract: FBS components in viral culture fluid interfere with the detection of specific antibody in immunized fish. In the present study, we developed a method to eliminate the interference of FBS using a model of red sea bream iridovirus (RSIV) vaccine. In an experiment using yellowtail antiserum against BSA, which is a major component of FBS in cell culture media, antibodies against BSA were completely absorbed via incubation of antiserum in 50% FBS at 25 degrees C for 1 h. In antibody detection, ELISA with the sera of yellowtail surviving RSIV-challenge, antibodies against RSIV were detected, but those against FBS disappeared via the pre-treatment of the sera. Therefore, antibodies against RSIV are effectively detected using ELISA with the pre-treatment of fish sera.
Type: article
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 - Hokkaido University