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Cholic acid accumulation and its diminution by short-chain fatty acids in bifidobacteria

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Title: Cholic acid accumulation and its diminution by short-chain fatty acids in bifidobacteria
Authors: Kurdi, Peter Browse this author
Tanaka, Hiroshi Browse this author
W. van Veen, Hendrik Browse this author
Asano, Kozo Browse this author →KAKEN DB
Tomita, Fusao Browse this author
Yokota, Atsushi6 Browse this author →KAKEN DB
Authors(alt): 横田, 篤6
Issue Date: 2003
Publisher: Society for General Microbiology
Journal Title: Microbiology
Volume: 149
Start Page: 2031
End Page: 2037
Publisher DOI: 10.1099/mic.0.26376-0
Abstract: Cholic acid (CA) transport was investigated in nine intestinal Bifidobacterium strains. Upon energization with glucose, all of the bifidobacteria accumulated CA. The driving force behind CA accumulation was found to be the transmembrane proton gradient (ΔpH, alkaline interior). The levels of accumulated CA generally coincided with the theoretical values, which were calculated by the Henderson–Hasselbalch equation using the measured internal pH values of the bifidobacteria, and a pKa value of 6・4 for CA. These results suggest that the mechanism of CA accumulation is based on the diffusion of a hydrophobic weak acid across the bacterial cell membrane, and its dissociation according to the ΔpH value. A mixture of short-chain fatty acids (acetate, propionate and butyrate) at the appropriate colonic concentration (117 mM in total) reduced CA accumulation in Bifidobacterium breve JCM 1192T. These short-chain fatty acids, which are weak acids, reduced the ΔpH, thereby decreasing CA accumulation in a dose-dependent manner. The bifidobacteria did not alter or modify the CA molecule. The probiotic potential of CA accumulation in vivo is discussed in relation to human bile acid metabolism.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 横田 篤

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