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DNA markers linked to Pga1, an adzuki bean gene that confers resistance to Cadophora gregata race 1
| Title: | DNA markers linked to Pga1, an adzuki bean gene that confers resistance to Cadophora gregata race 1 |
| Authors: | Suzuki, Takako Browse this author | | Yoshii, Takamitsu Browse this author | | Fujita, Shohei Browse this author | | Shimada, Hisanori Browse this author | | Takeuchi, Toru Browse this author | | Kondo, Norio Browse this author |
| Keywords: | adzuki bean | | brown stem rot | | DNA marker | | Cadophora gregata | | resistance gene |
| Issue Date: | Sep-2013 |
| Publisher: | Japanese soc breeding |
| Journal Title: | Breeding science |
| Volume: | 63 |
| Issue: | 3 |
| Start Page: | 353 |
| End Page: | 357 |
| Publisher DOI: | 10.1270/jsbbs.63.353 |
| Abstract: | Brown stem rot (BSR) caused by Cadophora gregata f. sp. adzukicola (syn. Phialophora gregata) is a serious soilborne disease of adzuki bean (Vigna angularis) in Japan. Cultivation of resistant cultivars is the most effective disease control method, therefore the selection of resistant lines is a priority for breeders. BSR-resistant adzuki bean lines have been screened in pathogen-infected fields. However, field selection using the pathogen and artificial inoculation methods is time-consuming and labor-intensive. In the present study, we used 105 F-3 lines derived from a cross between a BSR-resistant cultivar 'Syumari' and a susceptible cultivar 'Buchishoryukei-1' for BSR inoculation tests. Amplified fragment-length polymorphism (AFLP) analyses with 1024 primer sets revealed that six fragments were polymorphic between resistance and susceptible bulked groups. Five DNA markers (Pg77, Pg118, Pg138, Pg139 and Pg126) were developed from the nucleotide sequences of polymorphic AFLP markers and their flanking regions. Pg118, which was derived from E-ACT/M-ACT-118, was tightly linked to the resistance gene Pga1 and was converted into a codominant marker for its easier use in marker-assisted selection for adzuki bean BSR resistance. Finally, the applicability of the developed markers for BSR resistance was tested on 32 adzuki bean accessions or cultivars. |
| Relation: | https://www.jstage.jst.go.jp/browse/jsbbs |
| Type: | article |
| URI: | http://hdl.handle.net/2115/54501 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 近藤 則夫
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