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Visualization of Ras-PI3K interaction in the endosome using BiFC

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Title: Visualization of Ras-PI3K interaction in the endosome using BiFC
Authors: Tsutsumi, Kaori Browse this author →KAKEN DB
Fujioka, Yoichiro Browse this author
Tsuda, Masumi Browse this author →KAKEN DB
Kawaguchi, Hideaki Browse this author →KAKEN DB
Ohba, Yusuke Browse this author →KAKEN DB
Keywords: Ras
Issue Date: Nov-2009
Publisher: Elsevier
Journal Title: Cellular Signalling
Volume: 21
Issue: 11
Start Page: 1672
End Page: 1679
Publisher DOI: 10.1016/j.cellsig.2009.07.004
PMID: 19616621
Abstract: Recent studies indicate the importance of spatiotemporal regulation in the diversity and specificity of intracellular signaling. Here, we show that Ras-PI3K signaling plays an important role in the local regulation of phosphatidylinositol metabolism in the endosome through live-cell imaging by using a bimolecular fluorescence complementation technique, in which molecular interaction is indicated by fluorescence emission. Using several possible combinations of Ras and the Ras-binding domain, we identified an optimal set of probe molecules that yielded the most significant increase in fluorescence intensity between the active and inactive forms of Ras. This combination revealed that, among the Ras effectors tested, phosphatidylinositol 3-kinase (PI3K) was specifically implicated in signaling in the endosome. We also found that full length PI3K was recruited to the endosome in EGF- and Ras-dependent manners, which appears to be essential for the activation of PI3K in this compartment. Taken together, these findings demonstrate that the spatiotemporal regulation of Ras-PI3K signaling may dictate the activation of PI3K and subsequent downstream signaling in the endosome.
Type: article (author version)
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 大場 雄介

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