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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
Peer-reviewed Journal Articles, etc >
Transcription factor 8 activates R-Ras to regulate angiogenesis
| Title: | Transcription factor 8 activates R-Ras to regulate angiogenesis |
| Authors: | Inuzuka, Takayuki Browse this author | | Tsuda, Masumi Browse this author →KAKEN DB | | Kawaguchi, Hideaki Browse this author →KAKEN DB | | Ohba, Yusuke Browse this author →KAKEN DB |
| Keywords: | TCF8 | | R-Ras | | CalDAG-GEF | | Angiogenesis |
| Issue Date: | 6-Feb-2009 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 379 |
| Issue: | 2 |
| Start Page: | 510 |
| End Page: | 513 |
| Publisher DOI: | 10.1016/j.bbrc.2008.12.101 |
| PMID: | 19116136 |
| Abstract: | We have recently reported that transcription factor 8 (TCF8) negatively regulates pathological angiogenesis by regulating endothelial invasiveness by acting as a transcriptional attenuator of matrix metalloproteinase 1. TCF8 also modulates cell–matrix and cell–cell adhesion; however molecular mechanism of this TCF8 function remains obscure. Here, we provide evidence that TCF8 activates R-Ras, another class of angiogenic regulator, to suppress angiogenesis by a mechanism other than a transcriptional attenuator. Tube formation by human umbilical vein endothelial cells (HUVECs) facilitated by TCF8 suppression was significantly inhibited by the expression of onstitutive active mutant of R-Ras. When we examined the mRNA expression levels of R-Ras regulators, no significant changes were observed to explain the R-Ras activation by TCF8. Interestingly, we found that TCF8 bound to CalDAG-GEFIII, an R-Ras activator, in the cytosol, indicating that TCF8 emanates signaling for R-Ras activation from cytosol to regulate angiogenesis negatively. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/54566 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 大場 雄介
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