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Transcriptional factors associated with epithelial-mesenchymal transition in choroidal neovascularization.

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Title: Transcriptional factors associated with epithelial-mesenchymal transition in choroidal neovascularization.
Authors: Hirasawa, Manabu Browse this author
Noda, Kousuke Browse this author →KAKEN DB
Noda, Setsuko Browse this author
Suzuki, Misa Browse this author
Ozawa, Yoko Browse this author
Shinoda, Kei Browse this author
Inoue, Makoto Browse this author
Ogawa, Yoko Browse this author
Tsubota, Kazuo Browse this author
Ishida, Susumu Browse this author →KAKEN DB
Issue Date: 6-May-2011
Publisher: Molecular Vision
Journal Title: Molecular vision
Volume: 17
Start Page: 1222
End Page: 1230
PMID: 21617757
Abstract: Purpose: To investigate the transcriptional factors associated with epithelial-mesenchymal transition (EMT) in choroidal neovascularization (CNV) secondary to age-related macular degeneration (AMD). Methods: Paraffin sections of CNV obtained from patients with AMD (n=12) were stained for transcriptional factors related to EMT, i.e., Snail, Slug, SIP1, and Twist. As a control, postmortem sections of ocular normal tissue were used. Furthermore, using a human retinal pigment epithelial (RPE) cell line (ARPE-19), reverse transcription–polymerase chain reaction (RT–PCR) and immunofluorescence microscopy were performed to explore the cellular localization and expression levels of EMT-associated transcriptional factors upon cytokine stimulation. Results: Of 12 specimens, 11 CNV tissues (91.6%) showed staining for Snail localized in cellular nuclei, particularly in those of RPE cells. Snail was strongly co-localized with α-smooth muscle antigen (SMA) in RPE cells. In contrast, postmortem human retina showed no Snail staining in RPE cells. Other transcriptional factors, Slug, Twist and SIP1 were not detected in CNV or normal human retina. In ARPE-19 cells, RT–PCR and immunofluorescence microscopy showed that Snail mRNA was upregulated by transforming growth factor (TGF)-β and VEGF stimulation. Furthermore, TGF-β induced relocalization of Snail to the nucleus in RPE cells. Conclusions: The current data indicate that Snail is a major transcriptional factor for EMT changes of RPE cells in human CNV.
Type: article
URI: http://hdl.handle.net/2115/54620
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 石田 晋

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